Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column

	Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column
	Jiang, Xiaogang 1,2; Dong, Jing 1; Wang, Fangjun 1; Feng, Shun 1; Ye, Mingliang 1; Zou, Hanfa 1
刊名	electrophoresis
	2008-04-01
卷号	29 期号:8 页码:1612-1618
关键词	automation monolithic analytical capillary column nano-LC-MS/MS peptide separation sample introduction
ISSN号	0173-0835
通讯作者	邹汉法
产权排序	1;1
英文摘要	an automated nano-lc-ms/ms platform without trap column was established, which only used a 20 cm lauryl methacrylate-ethylene dimethacrylate (lma-edma) monolithic capillary column to allow preconcentration and separation of peptides. the monolithic column had the advantages of good permeability and low backpressure resulting in higher flow rates for capillary columns. tryptic digests of bovine albumin and yeast protein extract were tested using the monolithic column system. high proteomic coverage using this approach were demonstrated in this study. furthermore, peptide samples extracted from mouse liver were separated by using the monolithic column system combined with size-exclusion chromatography prefractionation. this monolithic column system might be a promising alternative for the automated system previously using a trap column for routine proteome and peptide profiling analysis.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	exchange trap column ; human serum proteome ; ionization emitter ; identification ; performance ; ms/ms ; proteins ; system ; electrochromatography ; preconcentration
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000255703100004
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/99997]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Suzhou Univ, Sch Med, Suzhou 215006, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Xiaogang,Dong, Jing,Wang, Fangjun,et al. Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column[J]. electrophoresis,2008,29(8):1612-1618.
APA	Jiang, Xiaogang,Dong, Jing,Wang, Fangjun,Feng, Shun,Ye, Mingliang,&Zou, Hanfa.(2008).Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column.electrophoresis,29(8),1612-1618.
MLA	Jiang, Xiaogang,et al."Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column".electrophoresis 29.8(2008):1612-1618.