STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS

	STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS
	Lin, F.1,2; Liu, Q. H.1; Xiao, Z. Z.1; Ma, D. Y.1; Xu, S. H.; Xiao, Y. S.; Li, J.1
刊名	CRYOLETTERS
	2011-07-01
卷号	32 期号:4 页码:339-348
关键词	Pagrus Major Dmso Permeability Capillary Electrophoresis Hatching Rate Morphology Changes
ISSN号	0143-2044
文献子类	Article
英文摘要	The objectives were to investigate the permeability of DMSO to red seabream (Pagrus major) embryos by capillary electrophoresis and the effects of DMSO concentrations (5%-40%, v/v) and immersion times (10, 30 and 60 min) on hatching rate and morphology. The results suggested the internal DMSO concentrations were positively related with the external concentrations and exposure times, while the hatching rate was negatively related. The hatching rate decreased drastically (< 50%) after exposure in 35%, 20% and 15% DMSO for over 10, 30 and 60 min, respectively. In all groups, when hatching rate was > 50%, the internal DMSO concentration was < 2%, which was still insufficient for successful cryopreservation. Morphological changes indicated the chorion was permeable to the cryoprotectant. A sign of dehydration in yolk were observed, for a significant decrease in the maximal yolk sac diameter. However, further research was needed to investigate whether the DMSO permeated into the yolk.; The objectives were to investigate the permeability of DMSO to red seabream (Pagrus major) embryos by capillary electrophoresis and the effects of DMSO concentrations (5%-40%, v/v) and immersion times (10, 30 and 60 min) on hatching rate and morphology. The results suggested the internal DMSO concentrations were positively related with the external concentrations and exposure times, while the hatching rate was negatively related. The hatching rate decreased drastically (< 50%) after exposure in 35%, 20% and 15% DMSO for over 10, 30 and 60 min, respectively. In all groups, when hatching rate was > 50%, the internal DMSO concentration was < 2%, which was still insufficient for successful cryopreservation. Morphological changes indicated the chorion was permeable to the cryoprotectant. A sign of dehydration in yolk were observed, for a significant decrease in the maximal yolk sac diameter. However, further research was needed to investigate whether the DMSO permeated into the yolk.
学科主题	Life Sciences & Biomedicine - Other Topics ; Physiology
语种	英语
WOS记录号	WOS:000295308200006
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12060]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lin, F.,Liu, Q. H.,Xiao, Z. Z.,et al. STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS[J]. CRYOLETTERS,2011,32(4):339-348.
APA	Lin, F..,Liu, Q. H..,Xiao, Z. Z..,Ma, D. Y..,Xu, S. H..,...&Li, J..(2011).STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS.CRYOLETTERS,32(4),339-348.
MLA	Lin, F.,et al."STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS".CRYOLETTERS 32.4(2011):339-348.