In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica

doi:10.1007/s00343-011-0181-9

	In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica
	Wang Jing1 ; Zhang Quanbin 1; Zhang Zhongshan 1,2; Hou Yun 1,2; Zhang Hong 1
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2011-05-01
卷号	29 期号:3 页码:679-685
关键词	Fucoidan Derivative Laminaria Japonica Anticoagulant Activity
ISSN号	0254-4059
DOI	10.1007/s00343-011-0181-9
文献子类	Article
英文摘要	Fucoidan, a group of sulfated heteropolysaccharides, was extracted from Laminaria japonica, an important economic alga species in China. The anticoagulant activity of fucoidan and its derivatives (including sulfated, phosphorylated, and aminated fucoidan) was examined using in-vitro anticoagulant systems. The correlation between chemical variations within the fucoidan group and anticoagulant activity was determined. The in-vitro anticoagulant properties of fucoidan and its derivatives were determined by measuring activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). The results indicate anticoagulant activity in all samples using APTT and TT assays; however, only the fucoidan derivatives affected the PT assay. Thus, the fucoidan derivatives were able to inhibit both intrinsic and extrinsic blood coagulants. Fucoidan (FPS) and its derivatives presented better anticoagulant activity than low molecular weight fucoidan (DFPS) and its derivatives, suggesting that molecular weight and proper conformation are contributing factors for anticoagulant activity of polysaccharides. Amino groups have a positive charge and can thus change the charge density of fucoidan. Accordingly, among the tested samples, aminated fucoidan (NF) was the most active reflecting the importance of charge density for anticoagulant activity. Available data obtained using in-vitro models suggest that the sulfate content, sulfate/total-sugar ratio, molecular weight, and the substituted group of fucoidan are important factors for anticoagulant activity but that the influence of sulfate, phosphate and amino groups on anticoagulant activity was different.; Fucoidan, a group of sulfated heteropolysaccharides, was extracted from Laminaria japonica, an important economic alga species in China. The anticoagulant activity of fucoidan and its derivatives (including sulfated, phosphorylated, and aminated fucoidan) was examined using in-vitro anticoagulant systems. The correlation between chemical variations within the fucoidan group and anticoagulant activity was determined. The in-vitro anticoagulant properties of fucoidan and its derivatives were determined by measuring activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). The results indicate anticoagulant activity in all samples using APTT and TT assays; however, only the fucoidan derivatives affected the PT assay. Thus, the fucoidan derivatives were able to inhibit both intrinsic and extrinsic blood coagulants. Fucoidan (FPS) and its derivatives presented better anticoagulant activity than low molecular weight fucoidan (DFPS) and its derivatives, suggesting that molecular weight and proper conformation are contributing factors for anticoagulant activity of polysaccharides. Amino groups have a positive charge and can thus change the charge density of fucoidan. Accordingly, among the tested samples, aminated fucoidan (NF) was the most active reflecting the importance of charge density for anticoagulant activity. Available data obtained using in-vitro models suggest that the sulfate content, sulfate/total-sugar ratio, molecular weight, and the substituted group of fucoidan are important factors for anticoagulant activity but that the influence of sulfate, phosphate and amino groups on anticoagulant activity was different.
学科主题	Marine & Freshwater Biology ; Oceanography
URL标识	查看原文
语种	英语
WOS记录号	WOS:000292558100023
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12024]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang Jing,Zhang Quanbin,Zhang Zhongshan,et al. In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2011,29(3):679-685.
APA	Wang Jing,Zhang Quanbin,Zhang Zhongshan,Hou Yun,&Zhang Hong.(2011).In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,29(3),679-685.
MLA	Wang Jing,et al."In-vitro anticoagulant activity of fucoidan derivatives from brown seaweed Laminaria japonica".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 29.3(2011):679-685.