Recognition of oxytocin by capillary electrochromatography with monolithic tetrapeptide-imprinted polymer used as the stationary phase

	Recognition of oxytocin by capillary electrochromatography with monolithic tetrapeptide-imprinted polymer used as the stationary phase
	Zheng, Chao ; Liu, Zhaosheng ; Gao, Ruyu ; Zhang, Lihua ; Zhang, Yukui
刊名	analytical and bioanalytical chemistry
	2007-07-01
卷号	388 期号:5-6 页码:1137-1145
关键词	molecularly imprinted polymer capillary electrochromatography monolithic column epitope approach oxytocin
通讯作者	张玉奎
产权排序	4;4
英文摘要	using yplg (tyr-pro-leu-gly), a tetrapeptide, as the template, an imprinted monolithic column was prepared and applied to the selective recognition of oxytocin based on the epitope approach and capillary electrochromatography (cec). by optimizing the polymerization solution in terms of functional monomer, cross-linking reagent, porogen, and imprinted template via cec evaluations of synthesized columns, an imprinted monolith with good recognition capacity (the imprinting factors for yplg and oxytocin were 4.499 and 4.013, respectively) and high column efficiency (theoretical plates for yplg and oxytocin were 22,995 plates/m and 16,952 plates/m, respectively) was achieved. in addition, the effects of various experimental parameters on the recognition of oxytocin, including the organic modifier content, the buffer concentration, and the ph value, were studied systematically. furthermore, a mixture of oxytocin and other proteins was analyzed using this monolithic cec column, and oxytocin was eluted much more slowly than other large biomolecules, which demonstrated the high selective recognition ability of such an imprinted monolith for oxytocin with plg (pro-leu-gly) as the epitope.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	molecular recognition ; epitope approach ; peptides ; separation ; media ; receptors ; proteins ; column ; acid
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000247998700019
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/99527]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R & A Ctr, Dalian 116023, Peoples R China 2.Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China 3.Tianjin Med Univ, Coll Pharm, Tianjin 300203, Peoples R China 4.Nankai Univ, Inst Element Organ Chem, State Key Lab Element Organ Chem, Tianjin 300071, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Chao,Liu, Zhaosheng,Gao, Ruyu,et al. Recognition of oxytocin by capillary electrochromatography with monolithic tetrapeptide-imprinted polymer used as the stationary phase[J]. analytical and bioanalytical chemistry,2007,388(5-6):1137-1145.
APA	Zheng, Chao,Liu, Zhaosheng,Gao, Ruyu,Zhang, Lihua,&Zhang, Yukui.(2007).Recognition of oxytocin by capillary electrochromatography with monolithic tetrapeptide-imprinted polymer used as the stationary phase.analytical and bioanalytical chemistry,388(5-6),1137-1145.
MLA	Zheng, Chao,et al."Recognition of oxytocin by capillary electrochromatography with monolithic tetrapeptide-imprinted polymer used as the stationary phase".analytical and bioanalytical chemistry 388.5-6(2007):1137-1145.