Cell-based high content screening using an integrated microfluidic device

	Cell-based high content screening using an integrated microfluidic device
	Ye, Nannan; Qin, Jianhua; Shi, Weiwei; Liu, Xin; Lin, Bingcheng
刊名	lab on a chip
	2007
卷号	7 期号:12 页码:1696-1704
通讯作者	林炳承 ; 秦建华
产权排序	1;1
英文摘要	high content screening (hcs) has quickly established itself as a core technique in the early stage of drug discovery for secondary compound screening. it allows several independent cellular parameters to be measured in a single cell or populations of cells in a single assay. in this work, we describe high content screening for the multiparametric measurement of cellular responses in human liver carcinoma (hepg2) cells using an integrated microfluidic device. this device consists of multiple drug gradient generators and parallel cell culture chambers, in which the processes of liquid dilution and diffusion, micro-scale cell culture, cell stimulation and cell labeling can be integrated into a single device. the simple assay provides multiparametric measurements of plasma membrane permeability, nuclear size, mitochondrial transmembrane potential and intracellular redox states in anti-cancer drug-induced apoptosis of hepg2 cells. the established platform is able to rapidly extract the maximum of information from tumor cells in response to several drugs varying in concentration, with minimal sample and less time, which is very useful for basic biomedical research and cancer treatment.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, multidisciplinary ; nanoscience & nanotechnology
研究领域[WOS]	biochemistry & molecular biology ; chemistry ; science & technology - other topics
关键词[WOS]	apoptosis ; systems ; array ; assay ; bioreactor ; generation ; gradients ; platform ; pathway ; culture
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000251121000018
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/99495]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Ye, Nannan,Qin, Jianhua,Shi, Weiwei,et al. Cell-based high content screening using an integrated microfluidic device[J]. lab on a chip,2007,7(12):1696-1704.
APA	Ye, Nannan,Qin, Jianhua,Shi, Weiwei,Liu, Xin,&Lin, Bingcheng.(2007).Cell-based high content screening using an integrated microfluidic device.lab on a chip,7(12),1696-1704.
MLA	Ye, Nannan,et al."Cell-based high content screening using an integrated microfluidic device".lab on a chip 7.12(2007):1696-1704.