Screening of phage displayed human liver cDNA library against dexamethasone

	Screening of phage displayed human liver cDNA library against dexamethasone
	Yu, Xiaoming 1,2; Zhao, Peng 1,2; Zhang, Weibing 1; Zhang, Lihua 1; Zhang, Yukui 1
刊名	journal of pharmaceutical and biomedical analysis
	2007-12-21
卷号	45 期号:5 页码:701-705
关键词	phage display cDNA library dexamethasone interaction
通讯作者	张丽华
产权排序	1;1
英文摘要	this paper described an attempt to establish a new method to screen the target biomolecule from phage displayed cdna library against small molecule drug insoluble in water. dexamethasone was selected as the model drug, and the screening was carried out in an eppendorf tube packed with the drug. the whole procedure was monitored by pcr with the enriched specific phage clone as the template. after four rounds screening, the pcr products of selected phages with the lengths of 400 and 600 bp were sequenced, and revealed identical sequences with cytochrome c oxidase subunit iii and albumin respectively by genbank searching. furthermore, frontal analysis-capillary electrophoresis (fa-ce) was performed to study the interaction between dexamethasone and albumin, and the binding constant was calculated to be 1.153 x 10(3), validating the weak specific interaction between the drug and the target protein. all these results demonstrated that with insoluble drug as the solid phase directly, the screening of target large molecule expressed in phage display cdna library was feasible, which might pave an easy way to screen the candidate drug targets. (c) 2007 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences ; life sciences & biomedicine
类目[WOS]	chemistry, analytical ; pharmacology & pharmacy
研究领域[WOS]	chemistry ; pharmacology & pharmacy
关键词[WOS]	capillary-electrophoresis ; protein interactions ; binding-protein ; expression ; identification ; selection ; cloning ; surface
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000252013300001
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/99465]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph Res & Anal Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Yu, Xiaoming,Zhao, Peng,Zhang, Weibing,et al. Screening of phage displayed human liver cDNA library against dexamethasone[J]. journal of pharmaceutical and biomedical analysis,2007,45(5):701-705.
APA	Yu, Xiaoming,Zhao, Peng,Zhang, Weibing,Zhang, Lihua,&Zhang, Yukui.(2007).Screening of phage displayed human liver cDNA library against dexamethasone.journal of pharmaceutical and biomedical analysis,45(5),701-705.
MLA	Yu, Xiaoming,et al."Screening of phage displayed human liver cDNA library against dexamethasone".journal of pharmaceutical and biomedical analysis 45.5(2007):701-705.