Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea

doi:10.1016/j.resmic.2010.02.003

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	Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea
	Zhu, Kailing 2; Pan, Hongmiao 2; Li, Jinhua 3; Yu-Zhang, Kui 4; Zhang, Sheng-Da 2; Zhang, Wen-Yan 2; Zhou, Ke 2; Yue, Haidong 2; Pan, Yongxin 3,5; Xiao, Tian 2,5
刊名	RESEARCH IN MICROBIOLOGY
	2010-05-01
卷号	161 期号:4 页码:276-283
关键词	Magnetotactic Bacteria Response To Light Pure Culture Magnetosome Phylogenetic Analysis
ISSN号	0923-2508
DOI	10.1016/j.resmic.2010.02.003
文献子类	Article
英文摘要	Magnetotactic bacteria (MTB) are ubiquitous in aquatic habitats. Because of their fastidious requirements for growth conditions, only very few axenic MTB cultures have been obtained worldwide. In this study, we report a novel marine magnetotactic spirillum axenic culture, designated as QH-2, isolated from the China Sea. It was able to grow in semi-solid or liquid chemically defined medium. The cells were amphitrichously flagellated and contained one single magnetosome chain with an average number of 16 magnetosomes per cell. Phosphate and lipid granules were also observed in the cells. Both rock magnetism and energy-dispersive X-ray spectroscopy characterizations indicated that the magnetosomes in QH-2 were single-domain magnetites (Fe(3)O(4)). QH-2 cells swam mostly in a straight line at a velocity of 20-50 mu m/s and occasionally changed to a helical motion. Unlike other magnetotactic spirilla. QH-2 cells responded to light illumination. As a consequence of illumination, the cells changed the direction in which they swam from parallel to the magnetic field to antiparallel. This response appears to be similar to the effect of an increase in [O(2)]. Analysis of the QH-2 16S rRNA sequence showed that it had greater than 11% sequence divergence from freshwater magnetotactic spirilla. Thus, the marine QH-2 strain seems to be both phylogenetically and magnetotactically distinct from the freshwater Magnetospirillum spp. studied previously. (C) 2010 Elsevier Masson SAS. All rights reserved.; Magnetotactic bacteria (MTB) are ubiquitous in aquatic habitats. Because of their fastidious requirements for growth conditions, only very few axenic MTB cultures have been obtained worldwide. In this study, we report a novel marine magnetotactic spirillum axenic culture, designated as QH-2, isolated from the China Sea. It was able to grow in semi-solid or liquid chemically defined medium. The cells were amphitrichously flagellated and contained one single magnetosome chain with an average number of 16 magnetosomes per cell. Phosphate and lipid granules were also observed in the cells. Both rock magnetism and energy-dispersive X-ray spectroscopy characterizations indicated that the magnetosomes in QH-2 were single-domain magnetites (Fe3O4). QH-2 cells swam mostly in a straight line at a velocity of 20-50 mu m/s and occasionally changed to a helical motion. Unlike other magnetotactic spirilla. QH-2 cells responded to light illumination. As a consequence of illumination, the cells changed the direction in which they swam from parallel to the magnetic field to antiparallel. This response appears to be similar to the effect of an increase in [O-2]. Analysis of the QH-2 16S rRNA sequence showed that it had greater than 11% sequence divergence from freshwater magnetotactic spirilla. Thus, the marine QH-2 strain seems to be both phylogenetically and magnetotactically distinct from the freshwater Magnetospirillum spp. studied previously. (C) 2010 Elsevier Masson SAS. All rights reserved.
学科主题	Microbiology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000279125600005
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5511]
专题	海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.CNRS, Chim Bacterienne Lab, Inst Biol Struct & Microbiol, UPR9043, F-13009 Marseille, France 2.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Key Lab Earths Deep Interior, Inst Geol & Geophys, Beijing 100029, Peoples R China 4.Univ Reims, Lab Microscopies & Etud Nanostruct, F-51687 Reims, France 5.CNRS, Lab Int Associe Biomineralizat & NanoStruct, F-13009 Marseille, France
推荐引用方式 GB/T 7714	Zhu, Kailing,Pan, Hongmiao,Li, Jinhua,et al. Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea[J]. RESEARCH IN MICROBIOLOGY,2010,161(4):276-283.
APA	Zhu, Kailing.,Pan, Hongmiao.,Li, Jinhua.,Yu-Zhang, Kui.,Zhang, Sheng-Da.,...&Wu, Long-Fei.(2010).Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea.RESEARCH IN MICROBIOLOGY,161(4),276-283.
MLA	Zhu, Kailing,et al."Isolation and characterization of a marine magnetotactic spirillum axenic culture QH-2 from an intertidal zone of the China Sea".RESEARCH IN MICROBIOLOGY 161.4(2010):276-283.