Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus

doi:10.1016/j.toxicon.2008.04.170

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	Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus
	Wang, Xiao-Jie 1,2; Yu, Ren-Cheng 1; Luo, Xuan 1; Zhou, Ming-Jiang 1; Lin, Xiang-Tian 3
刊名	TOXICON
	2008-07-01
卷号	52 期号:1 页码:55-61
关键词	Nassarius Semiplicatus Tetrodotoxin Bacteria Lc-ms Elisa 16s Rdna
ISSN号	0041-0101
DOI	10.1016/j.toxicon.2008.04.170
文献子类	Article
英文摘要	Bacteria isolated from a highly toxic sample of gastropod Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2 x 10(2) mouse unit (MU) Per gram Of tissue (wet weight). High concentration of tetrodotoxin (TTX) and its analogues (TTXs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography coupled with mass chromatography (LC-MS). Bacterial strains isolated from the digestive gland were cultured and screened for TTX with a competitive ELISA method. Tetrodotoxin was detected in a proportion of bacterial strains, but the toxin content was low. Partial 16S ribosomal DNA (rDNA) of the TTX-producing strains was then sequenced and compared with those published in the GenBank to tentatively identify the toxic strains. It was found that most of the toxic strains were closely affiliated with genus Vibrio, and the others were related to genus Shewanella, Marinomonas, Tenacibaculum and Aeromonas. These findings suggest that tetrodotoxin-producing bacteria might play an important role in tetrodotoxin accumulation/production in N. semiplicatus. (C) 2008 Elsevier Ltd. All rights reserved.; Bacteria isolated from a highly toxic sample of gastropod Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2 x 10(2) mouse unit (MU) Per gram Of tissue (wet weight). High concentration of tetrodotoxin (TTX) and its analogues (TTXs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography coupled with mass chromatography (LC-MS). Bacterial strains isolated from the digestive gland were cultured and screened for TTX with a competitive ELISA method. Tetrodotoxin was detected in a proportion of bacterial strains, but the toxin content was low. Partial 16S ribosomal DNA (rDNA) of the TTX-producing strains was then sequenced and compared with those published in the GenBank to tentatively identify the toxic strains. It was found that most of the toxic strains were closely affiliated with genus Vibrio, and the others were related to genus Shewanella, Marinomonas, Tenacibaculum and Aeromonas. These findings suggest that tetrodotoxin-producing bacteria might play an important role in tetrodotoxin accumulation/production in N. semiplicatus. (C) 2008 Elsevier Ltd. All rights reserved.
学科主题	Pharmacology & Pharmacy ; Toxicology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000258816500007
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5397]
专题	海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Hlth Inspect Agcy Lianyungang, Lianyungang 222006, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiao-Jie,Yu, Ren-Cheng,Luo, Xuan,et al. Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus[J]. TOXICON,2008,52(1):55-61.
APA	Wang, Xiao-Jie,Yu, Ren-Cheng,Luo, Xuan,Zhou, Ming-Jiang,&Lin, Xiang-Tian.(2008).Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus.TOXICON,52(1),55-61.
MLA	Wang, Xiao-Jie,et al."Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus".TOXICON 52.1(2008):55-61.