Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography

	Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography
	Wang, Yun 1,2,3; Kong, Liang 4; Hu, Lianghai 3; Lei, Xiaoyuan 3; Yang, Li 1; Chou, Guixin 1; Zou, Hanfa 3; Wang, Changhong 1; Bligh, S. W. Annie 1; Wang, Zhengtao 1,2
刊名	journal of chromatography b-analytical technologies in the biomedical and life sciences
	2007-12-15
卷号	860 期号:2 页码:185-194
关键词	comprehensive two-dimensional biochromatography Longdan Xiegan Decoction HSA column biological fingerprinting analysis traditional chinese medicine
ISSN号	1570-0232
通讯作者	邹汉法 ; zhengtao wang
产权排序	2;1
英文摘要	a comprehensive two-dimensional biochromatography method using a silica-bonded human serum albumin (hsa) column and a rp-hplc column was developed for the biological fingerprinting analysis of bioactive components in a traditional chinese medicine (tcm) prescription, longdan xiegan decoction (lxd). the biochromatography with hsa-immobilized stationary phase was applied to study the interaction of multiple components in lxd with hsa in the first dimension, and fractions of hsa column were further separated by a silica monolithic ods column (on-line)/an ods column (off-line) coupled with a diode array detector and an atmospheric pressure chemical ionization mass spectrometer (apci-ms). more than 100 compounds in lxd that interacted with the immobilized hsa were separated and analyzed. among them 19 compounds were identified based on their retention values, uv spectra, molecular weights and mass spectra. the results show that the developed comprehensive two-dimensional biochromatography system reported here is capable of being used for biological fingerprinting analysis of natural products in complex matrices such as extracts of tcms and their prescriptions. (c) 2007 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	2-dimensional liquid-chromatography ; tandem mass-spectrometry ; angelica-sinensis ; separation ; enantiomers ; geniposide ; extract ; protein ; plasma ; system
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000251919400007
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/99375]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Shanghai Univ Tradit Chinese Med, Minist Educ Res, Key Lab Stand Chinese Med, Shanghai 201203, Peoples R China 2.China Pharmaceut Univ, Dept Pharmacol, Nanjing 210038, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116023, Peoples R China 4.Dalian Fisheries Univ, Coll Marine Environm Engn, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yun,Kong, Liang,Hu, Lianghai,et al. Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2007,860(2):185-194.
APA	Wang, Yun.,Kong, Liang.,Hu, Lianghai.,Lei, Xiaoyuan.,Yang, Li.,...&Wang, Zhengtao.(2007).Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography.journal of chromatography b-analytical technologies in the biomedical and life sciences,860(2),185-194.
MLA	Wang, Yun,et al."Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography".journal of chromatography b-analytical technologies in the biomedical and life sciences 860.2(2007):185-194.