Structure-based function prediction of the expanding mollusk tyrosinase family

doi:10.1007/s00343-017-6066-9

	Structure-based function prediction of the expanding mollusk tyrosinase family
	Huang Ronglian1,2,4 ; Li Li 1,3; Zhang Guofan 1,3
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2017-11-01
卷号	35 期号:6 页码:1454-1464
关键词	Tyrosinase Mollusk Ligand Binding Pocket Substrate Diversity Evolution
DOI	10.1007/s00343-017-6066-9
文献子类	Article
英文摘要	Tyrosinase (Ty) is a common enzyme found in many different animal groups. In our previous study, genome sequencing revealed that the Ty family is expanded in the Pacific oyster (Crassostrea gigas). Here, we examine the larger number of Ty family members in the Pacific oyster by high-level structure prediction to obtain more information about their function and evolution, especially the unknown role in biomineralization. We verified 12 Ty gene sequences from Crassostrea gigas genome and Pinctada fucata martensii transcriptome. By using phylogenetic analysis of these Tys with functionally known Tys from other molluscan species, eight subgroups were identified (CgTy_s1, CgTy_s2, MolTy_s1, MolTy-s2, MolTy-s3, PinTy-s1, PinTy-s2 and PviTy). Structural data and surface pockets of the dinuclear copper center in the eight subgroups of molluscan Ty were obtained using the latest versions of prediction online servers. Structural comparison with other Ty proteins from the protein databank revealed functionally important residues (H-A1, H-A2, H-A3, H-B1, H-B2, H-B3, Z1-Z9) and their location within these protein structures. The structural and chemical features of these pockets which may related to the substrate binding showed considerable variability among mollusks, which undoubtedly defines Ty substrate binding. Finally, we discuss the potential driving forces of Ty family evolution in mollusks. Based on these observations, we conclude that the Ty family has rapidly evolved as a consequence of substrate adaptation in mollusks.
语种	英语
WOS记录号	WOS:000415810900016
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/143285]
专题	海洋研究所_海洋生物技术研发中心海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266235, Peoples R China 4.GuangDong Ocean Univ, Marine Pearl Culture Lab, Fishery Coll, Zhanjiang 524088, Peoples R China
推荐引用方式 GB/T 7714	Huang Ronglian,Li Li,Zhang Guofan. Structure-based function prediction of the expanding mollusk tyrosinase family[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(6):1454-1464.
APA	Huang Ronglian,Li Li,&Zhang Guofan.(2017).Structure-based function prediction of the expanding mollusk tyrosinase family.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(6),1454-1464.
MLA	Huang Ronglian,et al."Structure-based function prediction of the expanding mollusk tyrosinase family".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.6(2017):1454-1464.