High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention

doi:10.1016/j.aquaculture.2017.07.019

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	High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention
	Wang, Xueying1,2 ; Liu, Qinghua 1,2; Xiao, Yongshuang 1,2; Yang, Yang1,2,3 ; Wang, Yanfeng 1,2; Song, Zongcheng 4; You, Feng 1,2; An, Hao1,2,3 ; Li, Jun 1,2
刊名	AQUACULTURE
	2017-10-01
卷号	479 页码:808-816
关键词	Paralichthys Olivaceus High Temperature Germ Cell Sex Differentiation
DOI	10.1016/j.aquaculture.2017.07.019
文献子类	Article
英文摘要	Sex determination is not only resort to a single genetic cascade but also regulated along a continuum of environmental and heritable factors in many fish species. Temperature is one of the most important non-genetic sex differentiation elements. Previous studies regarding high temperature masculinization of flatfish typically focused on sex ratios and reproductive endocrinology, but little was known about germ cells during the process. In this study, we investigated the dynamic changes of germ cells in terms of morphology and number employing serial-sectioning and the molecular investigation of expression of dnd and amh genes by qRT-PCR for qualitative and quantitative analysis of germ cell proliferation during sexual differentiation. Moreover, the proliferation activity of primordial germ cells was investigated using PCNA (proliferating cell nuclear antigen) immunohistochemistry.
语种	英语
WOS记录号	WOS:000408034700099
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/143135]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋生物技术研发中心海洋研究所_胶州湾海洋生态系统国家野外研究站
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xueying,Liu, Qinghua,Xiao, Yongshuang,et al. High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention[J]. AQUACULTURE,2017,479:808-816.
APA	Wang, Xueying.,Liu, Qinghua.,Xiao, Yongshuang.,Yang, Yang.,Wang, Yanfeng.,...&Li, Jun.(2017).High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention.AQUACULTURE,479,808-816.
MLA	Wang, Xueying,et al."High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention".AQUACULTURE 479(2017):808-816.