Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida

doi:10.1111/jwas.12395

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida
	Du, Yishuai 1; Liu, Ying1 ; Xiao, Peng 1; Meng, Lingjie1,2 ; Liu, Pengfei 1,2
刊名	JOURNAL OF THE WORLD AQUACULTURE SOCIETY
	2017-08-01
卷号	48 期号:4 页码:574-582
关键词	Atlantic Salmon Aeromonas Salmonicida Real-time Pcr Sybr Green i Quantitative Detection
DOI	10.1111/jwas.12395
文献子类	Article
英文摘要	A rapid, economical, specific, and sensitive quantitative real-time polymerase chain reaction (qPCR) assay coupled with SYBR Green I chemistry was developed for the quantitative detection of Aeromonas salmonicida from farmed Atlantic salmon, Salmo salar, with the symptoms of furunculosis. The set of primers designed from the virulence array protein (vapA) gene was specific to A. salmonicida. Compared with the conventional PCR, qPCR had a lower detection limit of 5.6 copies of the positive plasmids. The standard curve, which showed the relationship between the copies of A. salmonicida and its quantification cycle (C-q) value, could be described as follows: log (copies of A. salmonicida)=-0.3213 C-q + 10.721. The quantitative detection of copies of A. salmonicida in different tissues of the moribund Atlantic salmon showed that A. salmonicida could be detected in all tissues; the spleen contained the largest number of A. salmonicida and then the kidney. These results suggest that the qPCR assay reported here is a specific, sensitive, and quantitative method for detecting A. salmonicida. It can be used for the routine tests of A. salmonicida in local aquaculture enterprise and for the research of infection routes of A. salmonicida to Atlantic salmon.
语种	英语
WOS记录号	WOS:000407075000005
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/143089]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Earth & Environm Sci Coll, Beijing 10049, Peoples R China
推荐引用方式 GB/T 7714	Du, Yishuai,Liu, Ying,Xiao, Peng,et al. Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2017,48(4):574-582.
APA	Du, Yishuai,Liu, Ying,Xiao, Peng,Meng, Lingjie,&Liu, Pengfei.(2017).Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,48(4),574-582.
MLA	Du, Yishuai,et al."Development and Application of a Quantitative Real-time Polymerase Chain Reaction Assay for the Detection of Aeromonas salmonicida".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 48.4(2017):574-582.