A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish

doi:10.1016/j.dci.2017.01.021

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish
	Li, Xue-peng1,2,3 ; Sun, Li 1,2
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2017-06-01
卷号	71 页码:49-58
关键词	Complement Factor b Cynoglossus Semilaevis Antimicrobial Bacterial Infection
DOI	10.1016/j.dci.2017.01.021
文献子类	Article
英文摘要	Complement factor B (Bf) is a component of the complement system. Following activation of the alternative pathway of the complement system, factor B is cleaved into Ba and Bb fragments. In fish, the Bf of rainbow trout is known to act as a C3 convertase, but the function of the Ba fragment is essentially unknown. In this study, we examined the expression patterns of tongue sole Cynoglossus semilaevis Bf (named CsBf) and the biological activity of the Ba fragment of CsBf (named CsBa). CsBf possesses the conserved domains of Bf and shares 39.9%-56.4% sequence identities With other fish Bf. CsBf expression was high in liver, muscle, and heart, and low in intestine, blood, and kidney. Bacterial infection significantly induced CsBf expression in kidney, spleen, and liver in a time-dependent manner. Recombinant CsBa (rCsBa) exhibited apparent binding capacities to bacteria and tongue sole peripheral blood leukocytes, and binding of rCsBa to bacteria inhibited bacterial growth. When overexpressed in tongue sole, CsBa significantly reduced bacterial dissemination in fish tissues. Together these results indicate for the first time that a fish Ba possesses antibacterial effect as well as immune cell-binding capacity, and thus probably plays a role in host immune defense against bacterial infection. (C) 2017 Elsevier Ltd. All rights reserved.
语种	英语
WOS记录号	WOS:000397834800006
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/136727]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Xue-peng,Sun, Li. A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,71:49-58.
APA	Li, Xue-peng,&Sun, Li.(2017).A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,71,49-58.
MLA	Li, Xue-peng,et al."A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 71(2017):49-58.