Salinity-induced oxidative stress and regulation of antioxidant defense system in the marine macroalga Ulva prolifera

doi:10.1016/j.jembe.2011.08.023

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	Salinity-induced oxidative stress and regulation of antioxidant defense system in the marine macroalga Ulva prolifera
	Luo, Min Bo 2; Liu, Feng 1
刊名	JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
	2011-12-01
卷号	409 期号:1-2 页码:223-228
关键词	Antioxidant Enzyme Catalase Oxidative Stress Salinity Ulva Prolifera
ISSN号	0022-0981
DOI	10.1016/j.jembe.2011.08.023
文献子类	Article
英文摘要	The physiological and biochemical responses to salinity stresses in Ulva prolifera were investigated, including growth rate, level of oxidative stress and regulation of antioxidant defense system. A six-day exposure to hyposaline (10 parts per thousand) and hypersaline (60 parts per thousand) conditions resulted in a significant decrease in growth rate and maximum photosynthetic quantum efficiency (Fv/Fm) compared with the control (30 parts per thousand). Increases in H(2)O(2) contents correlated to the level of lipid peroxidation, which suggested that oxidative damage occurred in salinity stress and was more severe at 60 parts per thousand than at 10 parts per thousand. The amount of total soluble protein (TSP) significantly increased in a hypersaline condition. The fluctuations of four antioxidant substrates and four antioxidant enzymes were determined after the long-term salinity stress. Compared to growth at 30 parts per thousand, low salinities led to a major increase in activities of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR), coupled with an increase in contents of ascorbate, glutathione and beta-carotenoid.Thalli exposed to hypersaline conditions rapidly accumulated glutathione and did not affect the content of ascorbate, alpha-tocopherol and beta-carotenoid. The activities of CAT, SOD, ascorbate peroxidase (APX) and GR increased in hypersaline conditions, suggesting that reactive oxygen scavenging enzymes played an important role in U. prolifera for adapting to the hypersaline condition. The alterations in antioxidant enzymes and substrates are not consistent between hyposaline and hypersaline stresses in U. prolifera, but the regulation of antioxidant defense system was a vital tolerance mechanism involved in the oxidative stress. (C) 2011 Elsevier B.V. All rights reserved.; The physiological and biochemical responses to salinity stresses in Ulva prolifera were investigated, including growth rate, level of oxidative stress and regulation of antioxidant defense system. A six-day exposure to hyposaline (10 parts per thousand) and hypersaline (60 parts per thousand) conditions resulted in a significant decrease in growth rate and maximum photosynthetic quantum efficiency (Fv/Fm) compared with the control (30 parts per thousand). Increases in H(2)O(2) contents correlated to the level of lipid peroxidation, which suggested that oxidative damage occurred in salinity stress and was more severe at 60 parts per thousand than at 10 parts per thousand. The amount of total soluble protein (TSP) significantly increased in a hypersaline condition. The fluctuations of four antioxidant substrates and four antioxidant enzymes were determined after the long-term salinity stress. Compared to growth at 30 parts per thousand, low salinities led to a major increase in activities of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR), coupled with an increase in contents of ascorbate, glutathione and beta-carotenoid.Thalli exposed to hypersaline conditions rapidly accumulated glutathione and did not affect the content of ascorbate, alpha-tocopherol and beta-carotenoid. The activities of CAT, SOD, ascorbate peroxidase (APX) and GR increased in hypersaline conditions, suggesting that reactive oxygen scavenging enzymes played an important role in U. prolifera for adapting to the hypersaline condition. The alterations in antioxidant enzymes and substrates are not consistent between hyposaline and hypersaline stresses in U. prolifera, but the regulation of antioxidant defense system was a vital tolerance mechanism involved in the oxidative stress. (C) 2011 Elsevier B.V. All rights reserved.
学科主题	Environmental Sciences & Ecology ; Marine & Freshwater Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000298363100028
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11934]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Minist Agr, Key & Open Lab Marine & Estuarine Fisheries Resou, Shanghai 200090, Peoples R China
推荐引用方式 GB/T 7714	Luo, Min Bo,Liu, Feng. Salinity-induced oxidative stress and regulation of antioxidant defense system in the marine macroalga Ulva prolifera[J]. JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY,2011,409(1-2):223-228.
APA	Luo, Min Bo,&Liu, Feng.(2011).Salinity-induced oxidative stress and regulation of antioxidant defense system in the marine macroalga Ulva prolifera.JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY,409(1-2),223-228.
MLA	Luo, Min Bo,et al."Salinity-induced oxidative stress and regulation of antioxidant defense system in the marine macroalga Ulva prolifera".JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY 409.1-2(2011):223-228.