Construction and analysis of the immune effect of an Edwardsiella tarda DNA vaccine encoding a D15-like surface antigen

doi:10.1016/j.fsi.2010.10.020

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	Construction and analysis of the immune effect of an Edwardsiella tarda DNA vaccine encoding a D15-like surface antigen
	Sun, Yun 1,2; Liu, Chun-Sheng 1,3; Sun, Li 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2011
卷号	30 期号:1 页码:273-279
关键词	Dna Vaccine Edwardsiella Tarda Immune Genes Macrophage Activation Passive Immunization
ISSN号	1050-4648
DOI	10.1016/j.fsi.2010.10.020
文献子类	Article
英文摘要	Edwardsiella tarda is a severe aquaculture pathogen with a broad host range that includes both humans and animal. In a previous study, we have identified in E. tarda a D15-like surface antigen, Esa1, which, when used as a recombinant subunit vaccine, is able to induce protective immunity in Japanese flounder (Paralichthys olivaceus) against E. tarda challenge. In this study, we examined further the immunoprotective potential of Esa1 as a DNA vaccine. For this purpose, the DNA vaccine plasmid pCEsa1 was constructed, which expresses esa1 under the cytomegalovirus immediate-early promoter. The vaccine potential of pCEsa1 was analyzed in the Japanese flounder model. The results showed that following vaccination, pCEsa1 and esa1 transcripts were detected in the muscle, liver, spleen, and kidney of the fish at 7, 21, and 49 days post-vaccination (p.v.). Production of Esa1 protein was also detected in the muscle tissue of pCEsa1-vaccinated fish. Compared to control fish, fish vaccinated with pCEsa1 exhibited significantly increased survival rates following E. tarda challenge at one and two months p.v.. Immunological analysis showed that vaccination with pCEsa1 (i) enhanced the respiratory burst activity, acid phosphatase activity, and bactericidal activity of head kidney macrophages; (ii) increased serum bactericidal activity in a Ca(2+)-independent manner; (iii) induced the production of specific serum antibodies, which became detectable at 3-week p.v. and afforded 57% protection (in terms of relative percent survival) upon naive fish as determined by passive immunization; (iv) upregulated the expression of a broad spectrum of genes possibly involved in both innate and adaptive immunity. These results indicate that pCEsa1 is an effective vaccine candidate against E. tarda and also provide insights to the immune mechanism of bacterial DNA vaccine. (C) 2010 Elsevier Ltd. All rights reserved.; Edwardsiella tarda is a severe aquaculture pathogen with a broad host range that includes both humans and animal. In a previous study, we have identified in E. tarda a D15-like surface antigen, Esa1, which, when used as a recombinant subunit vaccine, is able to induce protective immunity in Japanese flounder (Paralichthys olivaceus) against E. tarda challenge. In this study, we examined further the immunoprotective potential of Esa1 as a DNA vaccine. For this purpose, the DNA vaccine plasmid pCEsa1 was constructed, which expresses esa1 under the cytomegalovirus immediate-early promoter. The vaccine potential of pCEsa1 was analyzed in the Japanese flounder model. The results showed that following vaccination, pCEsa1 and esa1 transcripts were detected in the muscle, liver, spleen, and kidney of the fish at 7, 21, and 49 days post-vaccination (p.v.). Production of Esa1 protein was also detected in the muscle tissue of pCEsa1-vaccinated fish. Compared to control fish, fish vaccinated with pCEsa1 exhibited significantly increased survival rates following E. tarda challenge at one and two months p.v.. Immunological analysis showed that vaccination with pCEsa1 (i) enhanced the respiratory burst activity, acid phosphatase activity, and bactericidal activity of head kidney macrophages; (ii) increased serum bactericidal activity in a Ca(2+)-independent manner; (iii) induced the production of specific serum antibodies, which became detectable at 3-week p.v. and afforded 57% protection (in terms of relative percent survival) upon naive fish as determined by passive immunization; (iv) upregulated the expression of a broad spectrum of genes possibly involved in both innate and adaptive immunity. These results indicate that pCEsa1 is an effective vaccine candidate against E. tarda and also provide insights to the immune mechanism of bacterial DNA vaccine. (C) 2010 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识	查看原文
语种	英语
WOS记录号	WOS:000286905800034
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11820]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yun,Liu, Chun-Sheng,Sun, Li. Construction and analysis of the immune effect of an Edwardsiella tarda DNA vaccine encoding a D15-like surface antigen[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(1):273-279.
APA	Sun, Yun,Liu, Chun-Sheng,&Sun, Li.(2011).Construction and analysis of the immune effect of an Edwardsiella tarda DNA vaccine encoding a D15-like surface antigen.FISH & SHELLFISH IMMUNOLOGY,30(1),273-279.
MLA	Sun, Yun,et al."Construction and analysis of the immune effect of an Edwardsiella tarda DNA vaccine encoding a D15-like surface antigen".FISH & SHELLFISH IMMUNOLOGY 30.1(2011):273-279.