Comparative proteomic analysis of challenged Zhikong scallop (Chlamys farreri): A new insight into the anti-Vibrio immune response of marine bivalves

doi:10.1016/j.fsi.2011.10.014

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	Comparative proteomic analysis of challenged Zhikong scallop (Chlamys farreri): A new insight into the anti-Vibrio immune response of marine bivalves
	Huan, Pin; Wang, Hongxia; Liu, Baozhong
刊名	FISH & SHELLFISH IMMUNOLOGY
	2011-12-01
卷号	31 期号:6 页码:1186-1192
关键词	Chlamys Farreri Vibrio Hepatopancreas Proteome
ISSN号	1050-4648
DOI	10.1016/j.fsi.2011.10.014
文献子类	Article
英文摘要	The current studies on molecular mechanism of bivalves interacting with bacteria are mainly on mRNA and recombinant protein levels. These works provide little information on natural proteins, which limit further understandings. In this study, we conducted a pioneer work to promote researches on the anti-Vibrio immune response of Zhikong Scallop Chlamys farreri through proteomic techniques. Firstly a reference map was constructed for the hepatopancreas of C. farreri. Totally 65 protein spots were included in the reference map, while 46 of them were identified. Gene ontology analysis revealed high activities of metabolism and immunity in hepatopancreas. Furthermore, hepatopancreas of C. Jarred injected with Vibrio harveyi at 24 h post-injection (hpi) were used for comparative proteomic analysis. Totally 27 differentially expressed proteins spots after challenge were screened: and 15 were successfully identified. These proteins include some immune-related proteins, metabolism enzymes, and new molecules which were not paid attentions in previous immunity studies in C. farreri. The results indicated that molecular chaperons and the antioxidant system are key elements in the anti-Vibrio immune response of hepatopancreas of C. farreri. The identification of new molecules provides indications for further studies. The results of this work provide a new insight into the anti-Vibrio immune response of marine bivalves. (C) 2011 Published by Elsevier Ltd.; The current studies on molecular mechanism of bivalves interacting with bacteria are mainly on mRNA and recombinant protein levels. These works provide little information on natural proteins, which limit further understandings. In this study, we conducted a pioneer work to promote researches on the anti-Vibrio immune response of Zhikong Scallop Chlamys farreri through proteomic techniques. Firstly a reference map was constructed for the hepatopancreas of C. farreri. Totally 65 protein spots were included in the reference map, while 46 of them were identified. Gene ontology analysis revealed high activities of metabolism and immunity in hepatopancreas. Furthermore, hepatopancreas of C. Jarred injected with Vibrio harveyi at 24 h post-injection (hpi) were used for comparative proteomic analysis. Totally 27 differentially expressed proteins spots after challenge were screened: and 15 were successfully identified. These proteins include some immune-related proteins, metabolism enzymes, and new molecules which were not paid attentions in previous immunity studies in C. farreri. The results indicated that molecular chaperons and the antioxidant system are key elements in the anti-Vibrio immune response of hepatopancreas of C. farreri. The identification of new molecules provides indications for further studies. The results of this work provide a new insight into the anti-Vibrio immune response of marine bivalves. (C) 2011 Published by Elsevier Ltd.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识	查看原文
语种	英语
WOS记录号	WOS:000298569700058
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11806]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Huan, Pin,Wang, Hongxia,Liu, Baozhong. Comparative proteomic analysis of challenged Zhikong scallop (Chlamys farreri): A new insight into the anti-Vibrio immune response of marine bivalves[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(6):1186-1192.
APA	Huan, Pin,Wang, Hongxia,&Liu, Baozhong.(2011).Comparative proteomic analysis of challenged Zhikong scallop (Chlamys farreri): A new insight into the anti-Vibrio immune response of marine bivalves.FISH & SHELLFISH IMMUNOLOGY,31(6),1186-1192.
MLA	Huan, Pin,et al."Comparative proteomic analysis of challenged Zhikong scallop (Chlamys farreri): A new insight into the anti-Vibrio immune response of marine bivalves".FISH & SHELLFISH IMMUNOLOGY 31.6(2011):1186-1192.