Characterization of EST-SSR and genomic-SSR markers in the clam, Meretrix meretrix

doi:10.1007/s12686-011-9426-3

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Characterization of EST-SSR and genomic-SSR markers in the clam, Meretrix meretrix
	Lu, Xia 1,2; Wang, Hongxia 1; Dai, Ping 1,2; Liu, Baozhong1
刊名	CONSERVATION GENETICS RESOURCES
	2011-10-01
卷号	3 期号:4 页码:655-658
关键词	Est-ssr Genomic-ssr Microsatellite Clam Meretrix Meretrix
ISSN号	1877-7252
DOI	10.1007/s12686-011-9426-3
文献子类	Article
英文摘要	We described and characterized 17 expressed sequence tag (EST)-derived simple sequence repeats (SSR) and 16 genomic-derived SSRs for the clam Meretrix meretrix. The EST-SSRs comprised 17.6% dinucleotide repeats, 35.3% trinucleotide repeats, and 47.1% tetranucleotide repeats; whereas the majority of the G-SSRs were dinuleotide repeats (75.0%). The number of alleles for the 33 SSR markers varied from 6 to 15, with a mean of 10.3 alleles per locus. Observed and expected heterozygosity (Ho and He) varied from 0.613 to 0.824, and 0.687 to 0.850, respectively. Polymorphic information content (PIC) ranged from 0.645 to 0.842. The mean values of number of alleles, Ho, He, and PIC of G-SSRs were higher than those of the EST-SSRs. No significant deviations from Hardy-Weinberg equilibrium or linkage disequilibrium were observed in the 33 loci. These loci are the first to be characterized in M. meretrix and should be useful in the investigation of genetic evaluation for conservation.; We described and characterized 17 expressed sequence tag (EST)-derived simple sequence repeats (SSR) and 16 genomic-derived SSRs for the clam Meretrix meretrix. The EST-SSRs comprised 17.6% dinucleotide repeats, 35.3% trinucleotide repeats, and 47.1% tetranucleotide repeats; whereas the majority of the G-SSRs were dinuleotide repeats (75.0%). The number of alleles for the 33 SSR markers varied from 6 to 15, with a mean of 10.3 alleles per locus. Observed and expected heterozygosity (Ho and He) varied from 0.613 to 0.824, and 0.687 to 0.850, respectively. Polymorphic information content (PIC) ranged from 0.645 to 0.842. The mean values of number of alleles, Ho, He, and PIC of G-SSRs were higher than those of the EST-SSRs. No significant deviations from Hardy-Weinberg equilibrium or linkage disequilibrium were observed in the 33 loci. These loci are the first to be characterized in M. meretrix and should be useful in the investigation of genetic evaluation for conservation.
学科主题	Biodiversity & Conservation ; Genetics & Heredity
URL标识	查看原文
语种	英语
WOS记录号	WOS:000294682100014
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11794]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Lu, Xia,Wang, Hongxia,Dai, Ping,et al. Characterization of EST-SSR and genomic-SSR markers in the clam, Meretrix meretrix[J]. CONSERVATION GENETICS RESOURCES,2011,3(4):655-658.
APA	Lu, Xia,Wang, Hongxia,Dai, Ping,&Liu, Baozhong.(2011).Characterization of EST-SSR and genomic-SSR markers in the clam, Meretrix meretrix.CONSERVATION GENETICS RESOURCES,3(4),655-658.
MLA	Lu, Xia,et al."Characterization of EST-SSR and genomic-SSR markers in the clam, Meretrix meretrix".CONSERVATION GENETICS RESOURCES 3.4(2011):655-658.