A Dopa Decarboxylase Modulating the Immune Response of Scallop Chlamys farreri

doi:10.1371/journal.pone.0018596

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	A Dopa Decarboxylase Modulating the Immune Response of Scallop Chlamys farreri
	Zhou, Zhi 1,2; Yang, Jialong 1,2; Wang, Lingling 1; Zhang, Huan 1; Gao, Yang1,2 ; Shi, Xiaowei 1,2; Wang, Mengqiang1 ; Kong, Pengfei 1,2; Qiu, Limei 1; Song, Linsheng 1
刊名	PLOS ONE
	2011-04-13
卷号	6 期号:4 页码:e18596
ISSN号	1932-6203
DOI	10.1371/journal.pone.0018596
文献子类	Article
英文摘要	Background: Dopa decarboxylase (DDC) is a pyridoxal 5-phosphate (PLP)-dependent enzyme that catalyzes the decarboxylation of L-Dopa to dopamine, and involved in complex neuroendocrine-immune regulatory network. The function for DDC in the immunomodulation remains unclear in invertebrate.; Background: Dopa decarboxylase (DDC) is a pyridoxal 5-phosphate (PLP)-dependent enzyme that catalyzes the decarboxylation of L-Dopa to dopamine, and involved in complex neuroendocrine-immune regulatory network. The function for DDC in the immunomodulation remains unclear in invertebrate. Methodology: The full-length cDNA encoding DDC (designated CfDDC) was cloned from mollusc scallop Chlamys farreri. It contained an open reading frame encoding a polypeptide of 560 amino acids. The CfDDC mRNA transcripts could be detected in all the tested tissues, including the immune tissues haemocytes and hepatopancreas. After scallops were treated with LPS stimulation, the mRNA expression level of CfDDC in haemocytes increased significantly (5.5-fold, P<0.05) at 3 h and reached the peak at 12 h (9.8-fold, P<0.05), and then recovered to the baseline level. The recombinant protein of CfDDC (rCfDDC) was expressed in Escherichia coli BL21 (DE3)-Transetta, and 1 mg rCfDDC could catalyze the production of 1.651 +/- 0.22 ng dopamine within 1 h in vitro. When the haemocytes were incubated with rCfDDC-coated agarose beads, the haemocyte encapsulation to the beads was increased significantly from 70% at 6 h to 93% at 24 h in vitro in comparison with that in the control (23% at 6 h to 25% at 24 h), and the increased haemocyte encapsulation was repressed by the addition of rCfDDC antibody (which is acquired via immunization 6-week old rats with rCfDDC). After the injection of DDC inhibitor methyldopa, the ROS level in haemocytes of scallops was decreased significantly to 0.41-fold (P<0.05) of blank group at 12 h and 0.47-fold (P<0.05) at 24 h, respectively. Conclusions: These results collectively suggested that CfDDC, as a homologue of DDC in scallop, modulated the immune responses such as haemocytes encapsulation as well as the ROS level through its catalytic activity, functioning as an indispensable immunomodulating enzyme in the neuroendocrine-immune regulatory network of mollusc.
学科主题	Life Sciences & Biomedicine - Other Topics
URL标识	查看原文
语种	英语
WOS记录号	WOS:000289458800021
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11742]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋腐蚀与防护研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Zhi,Yang, Jialong,Wang, Lingling,et al. A Dopa Decarboxylase Modulating the Immune Response of Scallop Chlamys farreri[J]. PLOS ONE,2011,6(4):e18596.
APA	Zhou, Zhi.,Yang, Jialong.,Wang, Lingling.,Zhang, Huan.,Gao, Yang.,...&Song, Linsheng.(2011).A Dopa Decarboxylase Modulating the Immune Response of Scallop Chlamys farreri.PLOS ONE,6(4),e18596.
MLA	Zhou, Zhi,et al."A Dopa Decarboxylase Modulating the Immune Response of Scallop Chlamys farreri".PLOS ONE 6.4(2011):e18596.