Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing

doi:10.1016/j.dci.2010.07.008

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	Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing
	Xiao, Ping-ping 1,2; Hu, Yong-hua 1; Sun, Li 1
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2010-12-01
卷号	34 期号:12 页码:1237-1241
关键词	Cystatin b Scophthalmus Maximus Macrophage Bacterial Infection
ISSN号	0145-305X
DOI	10.1016/j.dci.2010.07.008
文献子类	Article
英文摘要	Cystatins form a large family of cysteine protease inhibitors found in a wide arrange of organisms. Studies have indicated that mammalian cystatins play important roles under both physiological and pathological conditions. However, much less is known about fish cystatins. In this report, we described the identification and analysis of a cystatin B homologue, SmCytB, from turbot Scophthalmus maximus. The open reading frame of SmCytB is 300 bp, which encodes a 99-residue protein that shares high levels of sequence identities with the cystatin B of a number of fish species and contains the conserved cysteine protease inhibitor motif of cystatin B. Constitutive expression of SmCytB is high in muscle, brain, heart and liver, and low in spleen. blood, gill and kidney. Bacterial infection upregulates SmCytB expression in kidney, spleen, liver and brain but not in muscle or heart. Functional analysis showed that recombinant SmCytB purified from Escherichia colt exhibits apparent cysteine protease inhibitor activity. Transient overexpression of SmCytB in head kidney macrophages enhances macrophage bactericidal activity probably through a nitric oxide-independent mechanism. These results indicate that SmCytB is involved in the immune defense of turbot against bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.; Cystatins form a large family of cysteine protease inhibitors found in a wide arrange of organisms. Studies have indicated that mammalian cystatins play important roles under both physiological and pathological conditions. However, much less is known about fish cystatins. In this report, we described the identification and analysis of a cystatin B homologue, SmCytB, from turbot Scophthalmus maximus. The open reading frame of SmCytB is 300 bp, which encodes a 99-residue protein that shares high levels of sequence identities with the cystatin B of a number of fish species and contains the conserved cysteine protease inhibitor motif of cystatin B. Constitutive expression of SmCytB is high in muscle, brain, heart and liver, and low in spleen. blood, gill and kidney. Bacterial infection upregulates SmCytB expression in kidney, spleen, liver and brain but not in muscle or heart. Functional analysis showed that recombinant SmCytB purified from Escherichia colt exhibits apparent cysteine protease inhibitor activity. Transient overexpression of SmCytB in head kidney macrophages enhances macrophage bactericidal activity probably through a nitric oxide-independent mechanism. These results indicate that SmCytB is involved in the immune defense of turbot against bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.
学科主题	Immunology ; Zoology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000283270700001
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5915]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Ping-ping,Hu, Yong-hua,Sun, Li. Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2010,34(12):1237-1241.
APA	Xiao, Ping-ping,Hu, Yong-hua,&Sun, Li.(2010).Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,34(12),1237-1241.
MLA	Xiao, Ping-ping,et al."Scophthalmus maximus cystatin B enhances head kidney macrophage-mediated bacterial killing".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 34.12(2010):1237-1241.