Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales, Phaeophyta)

doi:10.1007/s11427-008-0143-4

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	Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales, Phaeophyta)
	Zhang YiChen 1,2; Jiang Peng 1; Gao JiangTao 1,3; Liao JianMin 4; Sun ShiJing 4; Shen ZiLong 4; Qin Song 1
刊名	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
	2008-12-01
卷号	51 期号:12 页码:1116-1120
关键词	Laminaria Japonica Gametophyte Genetic Engineering Reteplase Rt-pa Bioreactor
ISSN号	1006-9305
DOI	10.1007/s11427-008-0143-4
文献子类	Article
英文摘要	The life cycle of seaweed Laminaria japonica involves a generation alternation between diploid sporophyte and haploid gametophte. The expression of foreign genes in sporophte has been proved. In this research, the recombinant expression in gametophyte was investigated by particle bombardment with the rt-PA gene encoding the recombinant human tissue-type plasminogen activator (Reteplase), which is a thrombolytic agent for acute myocardial infarction (AMI). Transgenic gametophytes were selected by their resistance to herbicide phosphiothricin (PPT), and proliferated in an established bubble column photo-bioreactor. According to the results from quantitative ELISA, Southern blotting, and fibrin agarose plate assay (FAPA) for bioactivity, it was showed that the rt-PA gene had been integrated into the genome of gametophytes of L. japonica, and the expression product showed the expected bioactivity, implying the proper post-transcript modification in haploid gametophyte.; The life cycle of seaweed Laminaria japonica involves a generation alternation between diploid sporophyte and haploid gametophte. The expression of foreign genes in sporophte has been proved. In this research, the recombinant expression in gametophyte was investigated by particle bombardment with the rt-PA gene encoding the recombinant human tissue-type plasminogen activator (Reteplase), which is a thrombolytic agent for acute myocardial infarction (AMI). Transgenic gametophytes were selected by their resistance to herbicide phosphiothricin (PPT), and proliferated in an established bubble column photo-bioreactor. According to the results from quantitative ELISA, Southern blotting, and fibrin agarose plate assay (FAPA) for bioactivity, it was showed that the rt-PA gene had been integrated into the genome of gametophytes of L. japonica, and the expression product showed the expected bioactivity, implying the proper post-transcript modification in haploid gametophyte.
学科主题	Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000261832500009
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5745]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tianjin Normal Univ, Coll Chem & Life Sci, Tianjin 300387, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.China Pharmaceut Univ, Ctr Biotechnol, Nanjing 210009, Peoples R China
推荐引用方式 GB/T 7714	Zhang YiChen,Jiang Peng,Gao JiangTao,et al. Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales, Phaeophyta)[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2008,51(12):1116-1120.
APA	Zhang YiChen.,Jiang Peng.,Gao JiangTao.,Liao JianMin.,Sun ShiJing.,...&Qin Song.(2008).Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales, Phaeophyta).SCIENCE IN CHINA SERIES C-LIFE SCIENCES,51(12),1116-1120.
MLA	Zhang YiChen,et al."Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales, Phaeophyta)".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 51.12(2008):1116-1120.