Molecular cloning and functional analysis of cathepsin B in nutrient metabolism during larval development in Meretrix meretrix | |
Wang, Xiaomei1,2; Liu, Baozhong1![]() ![]() | |
刊名 | AQUACULTURE
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2008-09-30 | |
卷号 | 282期号:1-4页码:41-46 |
关键词 | Meretrix Meretrix Larvae Cathepsin b Nutrient Metabolism |
ISSN号 | 0044-8486 |
DOI | 10.1016/j.aquaculture.2008.06.014 |
文献子类 | Article |
英文摘要 | Seed rearing is an important part in large scale clam culture industry. Since the nutritional history affects early development in bivalve, the condition of larval nutrition plays a key role in successful seed rearing. So far, the molecular mechanism of nutrient uptake in bivalve larvae is unclear. As one of the important proteolytic enzymes, cathepsin B of several organisms has been reported to be involved in digestion. We intended to analyze whether cathepsin B is involved in larval nutrient metabolism in the economic bivalve, clam Meretrix meretrix. The full length of M. meretrix cathepsin B (MmeCB) cDNA was cloned, which is 1647 bp with an open reading frame of 1014 bp. The deduced amino acid sequence encoded a preproenzyme of 337 residues with Cys-114, His-282 and Asn-302 composing cathepsin B activity center. The temporal and spatial expressions of MmeCB mRNA were examined from trochophore to post larva stages by whole mount in situ hybridization. In trochophore stage, no detectable signal was found. In the later three stages, MmeCB mRNA was detected in the digestive gland, suggesting a possible role of MmeCB in digestion. Moreover, MmeCB mRNA was also observed in the epidermal cells in D-veligers. Cathepsin B specific inhibitor (CA074 methyl ester) was applied to block the activity of cathepsin B in unfed larvae. The average shell lengths of treated larvae were smaller than that in control groups. The results of mRNA epidermal distribution and inhibitor treatment in D-veligers indicated that MmeCB may be also associated with other pathway of nutrient metabolism in larval epidermis. The overall results in this paper revealed that MmeCB might play a role in larval nutrient metabolism. (C) 2008 Elsevier B.V. All rights reserved.; Seed rearing is an important part in large scale clam culture industry. Since the nutritional history affects early development in bivalve, the condition of larval nutrition plays a key role in successful seed rearing. So far, the molecular mechanism of nutrient uptake in bivalve larvae is unclear. As one of the important proteolytic enzymes, cathepsin B of several organisms has been reported to be involved in digestion. We intended to analyze whether cathepsin B is involved in larval nutrient metabolism in the economic bivalve, clam Meretrix meretrix. The full length of M. meretrix cathepsin B (MmeCB) cDNA was cloned, which is 1647 bp with an open reading frame of 1014 bp. The deduced amino acid sequence encoded a preproenzyme of 337 residues with Cys-114, His-282 and Asn-302 composing cathepsin B activity center. The temporal and spatial expressions of MmeCB mRNA were examined from trochophore to post larva stages by whole mount in situ hybridization. In trochophore stage, no detectable signal was found. In the later three stages, MmeCB mRNA was detected in the digestive gland, suggesting a possible role of MmeCB in digestion. Moreover, MmeCB mRNA was also observed in the epidermal cells in D-veligers. Cathepsin B specific inhibitor (CA074 methyl ester) was applied to block the activity of cathepsin B in unfed larvae. The average shell lengths of treated larvae were smaller than that in control groups. The results of mRNA epidermal distribution and inhibitor treatment in D-veligers indicated that MmeCB may be also associated with other pathway of nutrient metabolism in larval epidermis. The overall results in this paper revealed that MmeCB might play a role in larval nutrient metabolism. (C) 2008 Elsevier B.V. All rights reserved. |
学科主题 | Fisheries ; Marine & Freshwater Biology |
URL标识 | 查看原文 |
语种 | 英语 |
WOS记录号 | WOS:000260292000007 |
公开日期 | 2010-12-24 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/5677] ![]() |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Xiaomei,Liu, Baozhong,Wang, Guodong,et al. Molecular cloning and functional analysis of cathepsin B in nutrient metabolism during larval development in Meretrix meretrix[J]. AQUACULTURE,2008,282(1-4):41-46. |
APA | Wang, Xiaomei,Liu, Baozhong,Wang, Guodong,Tang, Baojun,&Xiang, Jianhai.(2008).Molecular cloning and functional analysis of cathepsin B in nutrient metabolism during larval development in Meretrix meretrix.AQUACULTURE,282(1-4),41-46. |
MLA | Wang, Xiaomei,et al."Molecular cloning and functional analysis of cathepsin B in nutrient metabolism during larval development in Meretrix meretrix".AQUACULTURE 282.1-4(2008):41-46. |
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