Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio

doi:10.1007/s10126-008-9105-x

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	Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio
	Wang, Bing 1; Li, Fuhua 1; Luan, Wei 1,2; Xie, Yusu 1,2; Zhang, Chengsong1,2 ; Luo, Zhan 1,2; Gui, Lang 1,2; Yan, Hui 1,2; Xiang, Jianhai1
刊名	MARINE BIOTECHNOLOGY
	2008-12-01
卷号	10 期号:6 页码:664-675
关键词	Wssv Vibrio Cdna Microarray Fenneropenaeus Chinensis
ISSN号	1436-2228
DOI	10.1007/s10126-008-9105-x
文献子类	Article
英文摘要	Microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by WSSV and heat-inactivated Vibrio anguillarum, respectively. At 6 h post challenge (HPC), a total of 806 clones showed differential expression profile in WSSV-challenged samples, but not in Vibrio-challenged samples. The genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post WSSV-challenged (HPC-WSSV) tissues. However, a total of 155 clones showed differential expression in the Vibrio-challenged samples, but not in WSSV-challenged samples. Serine-type endopeptidase and lysosome-related genes were the most upregulated elements in tissues 6 h post Vibrio challenge (HPC-Vibrio). Totally, 188 clones showed differential expression in both 6 and 12 HPC-WSSV and HPC-Vibrio samples. Most of the differentially expressed genes (185/188) were downregulated in the samples of 12 HPC-WSSV, whereas upregulated in the samples at 6 and 12 HPC-Vibrio and 6 HPC-WSSV. The expression profiles of three differentially expressed genes identified in microarray hybridization were analyzed in hemocytes, lymphoid organ, and hepatopancreas of shrimp challenged by WSSV or Vibrio through real-time PCR. The results further confirmed the microarray hybridization results. The data will provide great help for us in understanding the immune mechanism of shrimp responding to WSSV or Vibrio.; Microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by WSSV and heat-inactivated Vibrio anguillarum, respectively. At 6 h post challenge (HPC), a total of 806 clones showed differential expression profile in WSSV-challenged samples, but not in Vibrio-challenged samples. The genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post WSSV-challenged (HPC-WSSV) tissues. However, a total of 155 clones showed differential expression in the Vibrio-challenged samples, but not in WSSV-challenged samples. Serine-type endopeptidase and lysosome-related genes were the most upregulated elements in tissues 6 h post Vibrio challenge (HPC-Vibrio). Totally, 188 clones showed differential expression in both 6 and 12 HPC-WSSV and HPC-Vibrio samples. Most of the differentially expressed genes (185/188) were downregulated in the samples of 12 HPC-WSSV, whereas upregulated in the samples at 6 and 12 HPC-Vibrio and 6 HPC-WSSV. The expression profiles of three differentially expressed genes identified in microarray hybridization were analyzed in hemocytes, lymphoid organ, and hepatopancreas of shrimp challenged by WSSV or Vibrio through real-time PCR. The results further confirmed the microarray hybridization results. The data will provide great help for us in understanding the immune mechanism of shrimp responding to WSSV or Vibrio.
学科主题	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000260838600005
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5661]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wang, Bing,Li, Fuhua,Luan, Wei,et al. Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio[J]. MARINE BIOTECHNOLOGY,2008,10(6):664-675.
APA	Wang, Bing.,Li, Fuhua.,Luan, Wei.,Xie, Yusu.,Zhang, Chengsong.,...&Xiang, Jianhai.(2008).Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio.MARINE BIOTECHNOLOGY,10(6),664-675.
MLA	Wang, Bing,et al."Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio".MARINE BIOTECHNOLOGY 10.6(2008):664-675.