Identification of a novel DNA methyltransferase 2 from the brine shrimp, Artemia franciscana

doi:10.1016/j.cbpb.2007.01.024

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	Identification of a novel DNA methyltransferase 2 from the brine shrimp, Artemia franciscana
	Feng, Chen-Zhuo ; Zhu, Xiao-Jing ; Dai, Zhong-Min ; Liu, Feng-Qi ; Xiang, Jian-Hai; Yang, Wei-Jun
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
	2007-06-01
卷号	147 期号:2 页码:191-198
关键词	Dnmt2 Artemia Franciscana Diapause Cysts Development
ISSN号	1096-4959
DOI	10.1016/j.cbpb.2007.01.024
文献子类	Article
英文摘要	DNA methyltransferase 2 (Dnmt2) is a dual-specificity DNA methyltransferase, which contains a weak DNA methyltransferase and novel tRNA methyltransferase activity. However, its biological function is still enigmatic. To elucidate the expression profiles of Dnmt2 in Artemia franciscana, we isolated the gene encoding a Dnmt2 from A. franciscana and named it as AfDnmt2. The cDNA of AfDnmt2 contained a 1140-bp open reading frame that encoded a putative Dnmt2 protein of 379 amino acids exhibiting 32%similar to 39% identities with other known Dnmt2 homologs. This is the first report of a DNA methyltransferase gene in Crustacean. By using semi-quantitative RT-PCR, A)Dnmt2 was found to be expressed through all developmental stages and its expression increased during resumption of diapause cysts development. Southern blot analysis indicated the presence of multiple copies of AfDnmt2 genes in A. franciscana. (C) 2007 Published by Elsevier Inc.; DNA methyltransferase 2 (Dnmt2) is a dual-specificity DNA methyltransferase, which contains a weak DNA methyltransferase and novel tRNA methyltransferase activity. However, its biological function is still enigmatic. To elucidate the expression profiles of Dnmt2 in Artemia franciscana, we isolated the gene encoding a Dnmt2 from A. franciscana and named it as AfDnmt2. The cDNA of AfDnmt2 contained a 1140-bp open reading frame that encoded a putative Dnmt2 protein of 379 amino acids exhibiting 32%similar to 39% identities with other known Dnmt2 homologs. This is the first report of a DNA methyltransferase gene in Crustacean. By using semi-quantitative RT-PCR, A)Dnmt2 was found to be expressed through all developmental stages and its expression increased during resumption of diapause cysts development. Southern blot analysis indicated the presence of multiple copies of AfDnmt2 genes in A. franciscana. (C) 2007 Published by Elsevier Inc.
学科主题	Biochemistry & Molecular Biology ; Zoology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000246544100006
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5543]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Zhejiang Univ, Inst Cell Biol & Genet, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China 2.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Feng, Chen-Zhuo,Zhu, Xiao-Jing,Dai, Zhong-Min,et al. Identification of a novel DNA methyltransferase 2 from the brine shrimp, Artemia franciscana[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2007,147(2):191-198.
APA	Feng, Chen-Zhuo,Zhu, Xiao-Jing,Dai, Zhong-Min,Liu, Feng-Qi,Xiang, Jian-Hai,&Yang, Wei-Jun.(2007).Identification of a novel DNA methyltransferase 2 from the brine shrimp, Artemia franciscana.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,147(2),191-198.
MLA	Feng, Chen-Zhuo,et al."Identification of a novel DNA methyltransferase 2 from the brine shrimp, Artemia franciscana".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 147.2(2007):191-198.