Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun

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	Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun
	Liu, ZY ; Xiang, JH; Zhou, GY ; Gong, ZX
刊名	CHINESE SCIENCE BULLETIN
	2001-05-01
卷号	46 期号:9 页码:766-770
关键词	Gene Gun Gfp Transgenic Shrimps
ISSN号	1001-6538
文献子类	Article
英文摘要	Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different development stages were observed with a fluorescent microscope. The transient expression of GFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry.; Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different development stages were observed with a fluorescent microscope. The transient expression of GFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry.
学科主题	Multidisciplinary Sciences
语种	英语
WOS记录号	WOS:000169054600017
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3315]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Liu, ZY,Xiang, JH,Zhou, GY,et al. Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun[J]. CHINESE SCIENCE BULLETIN,2001,46(9):766-770.
APA	Liu, ZY,Xiang, JH,Zhou, GY,&Gong, ZX.(2001).Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun.CHINESE SCIENCE BULLETIN,46(9),766-770.
MLA	Liu, ZY,et al."Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun".CHINESE SCIENCE BULLETIN 46.9(2001):766-770.