Analysis of three marine fish cell lines by RAPD assay

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Analysis of three marine fish cell lines by RAPD assay
	Guo, HR ; Zhang, SC ; Tong, SL ; Xiang, JH
刊名	IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
	2001-07-01
卷号	37 期号:7 页码:430-433
关键词	Fish Cell Line Characterization Genetic Variation Random Amplified Polymorphic Dna
ISSN号	1071-2690
文献子类	Article
英文摘要	We tested the applicability of the random amplified polymorphic deoxyribonucleic acid (RAPD) analysis for identification of three marine fish cell lines FG. SPH, and RSBK and as a possible tool to detect cross-contamination. Sixth commercial 10-mer RAPD primers were tested on the cell lines and on samples collected from individual fish. The results obtained showed that the cell lines could be identified to the correspondent species on the basis of identical Patterns produced by 35-48% of the primers tested. the total mean similarity indices for cell lines versus correspondent species of individual fish ranged from 0.825 to 0.851. indicating the existence of genetic variation in these cell lines in relation to the species of their origin. Also, four primers, which gave a monomorphic hand pattern within species/line, but different among the species/line, were obtained. These primers can be useful for identification of these cell lines and for characterization of the genetic variation of these cell lines in relation to the species of their origin. This supported the use of RAPD analysis as an effective tool in species identification and cross-contamination test among different cell lines.; We tested the applicability of the random amplified polymorphic deoxyribonucleic acid (RAPD) analysis for identification of three marine fish cell lines FG. SPH, and RSBK and as a possible tool to detect cross-contamination. Sixth commercial 10-mer RAPD primers were tested on the cell lines and on samples collected from individual fish. The results obtained showed that the cell lines could be identified to the correspondent species on the basis of identical Patterns produced by 35-48% of the primers tested. the total mean similarity indices for cell lines versus correspondent species of individual fish ranged from 0.825 to 0.851. indicating the existence of genetic variation in these cell lines in relation to the species of their origin. Also, four primers, which gave a monomorphic hand pattern within species/line, but different among the species/line, were obtained. These primers can be useful for identification of these cell lines and for characterization of the genetic variation of these cell lines in relation to the species of their origin. This supported the use of RAPD analysis as an effective tool in species identification and cross-contamination test among different cell lines.
学科主题	Cell Biology ; Developmental Biology
语种	英语
WOS记录号	WOS:000171006300007
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3313]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Ocean Univ Qingdao, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Guo, HR,Zhang, SC,Tong, SL,et al. Analysis of three marine fish cell lines by RAPD assay[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2001,37(7):430-433.
APA	Guo, HR,Zhang, SC,Tong, SL,&Xiang, JH.(2001).Analysis of three marine fish cell lines by RAPD assay.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,37(7),430-433.
MLA	Guo, HR,et al."Analysis of three marine fish cell lines by RAPD assay".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 37.7(2001):430-433.