Isolation and purification of phycoerythrin from red alga Garcilaria verrucosa by expanded-bed-adsorption and ion-exchange chromatogaphy | |
Wang, GC | |
刊名 | CHROMATOGRAPHIA |
2002-10-01 | |
卷号 | 56期号:7-8页码:509-513 |
关键词 | Column Liquid Chromatography Ion-exchange Chromatography Marine Algae Gracilaria Verrucosa R-phycoerythrin |
ISSN号 | 0009-5893 |
文献子类 | Article |
英文摘要 | R-phycoerythrin was isolated and purified from Gracilaria verrucosa on an expanded-bed adsorption column combined with ion-exchange chromatography, which can effectively solve the problem of blockage of chromatographic columns due to polysaccharides during isolation and purification of phycobiliproteins. 0.1 M (NH4)(2)SO4 proved best to elute R-phycoerythrin from the expanded-bed column, and desalted 0.1 M (NH4)(2)SO4 eluate was used on an ion-exchange column to purify the R-phycoerythrin. Using this two-stage chromatography, the purity (OD565/OD280) of the R-phycoerythrin from G. verrucosa is increased to 4.4, and the yield of purified R-phycoerythrin can reach 0.141 mg . g(-1) of the frozen alga.; R-phycoerythrin was isolated and purified from Gracilaria verrucosa on an expanded-bed adsorption column combined with ion-exchange chromatography, which can effectively solve the problem of blockage of chromatographic columns due to polysaccharides during isolation and purification of phycobiliproteins. 0.1 M (NH4)(2)SO4 proved best to elute R-phycoerythrin from the expanded-bed column, and desalted 0.1 M (NH4)(2)SO4 eluate was used on an ion-exchange column to purify the R-phycoerythrin. Using this two-stage chromatography, the purity (OD565/OD280) of the R-phycoerythrin from G. verrucosa is increased to 4.4, and the yield of purified R-phycoerythrin can reach 0.141 mg . g(-1) of the frozen alga. |
学科主题 | Biochemical Research Methods ; Chemistry, Analytical |
语种 | 英语 |
WOS记录号 | WOS:000178836600018 |
公开日期 | 2010-12-22 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/3293] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
作者单位 | Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, GC. Isolation and purification of phycoerythrin from red alga Garcilaria verrucosa by expanded-bed-adsorption and ion-exchange chromatogaphy[J]. CHROMATOGRAPHIA,2002,56(7-8):509-513. |
APA | Wang, GC.(2002).Isolation and purification of phycoerythrin from red alga Garcilaria verrucosa by expanded-bed-adsorption and ion-exchange chromatogaphy.CHROMATOGRAPHIA,56(7-8),509-513. |
MLA | Wang, GC."Isolation and purification of phycoerythrin from red alga Garcilaria verrucosa by expanded-bed-adsorption and ion-exchange chromatogaphy".CHROMATOGRAPHIA 56.7-8(2002):509-513. |
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