Construction and characterization of a bacterial artificial chromosome library of marine macroalga Porphyra yezoensis (Rhodophyta)

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	Construction and characterization of a bacterial artificial chromosome library of marine macroalga Porphyra yezoensis (Rhodophyta)
	Deng, DY ; Zhao, G ; Xuan, JS ; Yang, JL ; Duan, D ; Weng, ML ; Wang, B
刊名	PLANT MOLECULAR BIOLOGY REPORTER
	2004-12-01
卷号	22 期号:4 页码:375-386
关键词	Bac Library Gene Cloning Genomic Research Porphyra Yezoensis Trehalose-6-phosphate Synthase (Tps) Gene
ISSN号	0735-9640
文献子类	Article
英文摘要	A large-DNA-fragment library is necessary for research into the Porphyra genome. In this study, a bacterial artificial chromosome (BAC) library of Porphyra yezoensis was constructed and characterized. The library contains 54,144 BAC clones with an average insert size of about 65 kb and fewer than 0.7% of clones without large inserts. Therefore, its capacity is more than 6.6 P. yezoensis genome equivalents, and the probability of recovering any nuclear DNA sequence from the library is higher than 99%. The library shows good fidelity and stability. A putative trehalose-6-phosphate synthase (TPS) gene was successfully screened out from the library. The above results show that the library is useful for gene cloning and genomic research in P. yezoensis.; A large-DNA-fragment library is necessary for research into the Porphyra genome. In this study, a bacterial artificial chromosome (BAC) library of Porphyra yezoensis was constructed and characterized. The library contains 54,144 BAC clones with an average insert size of about 65 kb and fewer than 0.7% of clones without large inserts. Therefore, its capacity is more than 6.6 P. yezoensis genome equivalents, and the probability of recovering any nuclear DNA sequence from the library is higher than 99%. The library shows good fidelity and stability. A putative trehalose-6-phosphate synthase (TPS) gene was successfully screened out from the library. The above results show that the library is useful for gene cloning and genomic research in P. yezoensis.
学科主题	Biochemical Research Methods ; Plant Sciences
语种	英语
WOS记录号	WOS:000227914500005
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3189]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Laiyang Agr Coll, Laiyang 265200, Peoples R China
推荐引用方式 GB/T 7714	Deng, DY,Zhao, G,Xuan, JS,et al. Construction and characterization of a bacterial artificial chromosome library of marine macroalga Porphyra yezoensis (Rhodophyta)[J]. PLANT MOLECULAR BIOLOGY REPORTER,2004,22(4):375-386.
APA	Deng, DY.,Zhao, G.,Xuan, JS.,Yang, JL.,Duan, D.,...&Wang, B.(2004).Construction and characterization of a bacterial artificial chromosome library of marine macroalga Porphyra yezoensis (Rhodophyta).PLANT MOLECULAR BIOLOGY REPORTER,22(4),375-386.
MLA	Deng, DY,et al."Construction and characterization of a bacterial artificial chromosome library of marine macroalga Porphyra yezoensis (Rhodophyta)".PLANT MOLECULAR BIOLOGY REPORTER 22.4(2004):375-386.