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	The preparation and bioactivity research of agaro-oligosaccharides
	Chen, HM; Zheng, L; Yan, XJ
刊名	FOOD TECHNOLOGY AND BIOTECHNOLOGY
	2005
卷号	43期号:1页码:29-36
关键词	Agaro-oligosaccharides Orthogonal Matrix Hplc Acid Degradation Antioxidant Alpha-glucosidase
ISSN号	1330-9862
文献子类	Article
英文摘要	Agaro-oligosaccharides were hydrolytically obtained from agar using hydrochloric acid, citric acid, and cationic exchange resin (solid acid). The FT-IR and NMR data showed that the hydrolysate has the structure of agaro-oligomers. Orthogonal matrix method was applied to optimize the preparation conditions based on alpha-naphthylamine end-labeled HPLC analysis method. The optimal way for oligosaccharides with different degree of polymerization (DP) was achieved by using solid acid degradation, which could give high yield and avoid solution neutralization process. Agaro-oligosaccharides with high purity were consequently obtained by activated carbon column isolation. Furthermore, the antioxidant and alpha-glucosidase inhibitory activity of three fractions were also investigated. The result indicated that 8% ethanol-eluted fraction showed highest activity against alpha-glucosidase with IC50 of 8.84 mg/mL, while 25% ethanol-eluted fraction possessed excellent antioxidant ability.; Agaro-oligosaccharides were hydrolytically obtained from agar using hydrochloric acid, citric acid, and cationic exchange resin (solid acid). The FT-IR and NMR data showed that the hydrolysate has the structure of agaro-oligomers. Orthogonal matrix method was applied to optimize the preparation conditions based on alpha-naphthylamine end-labeled HPLC analysis method. The optimal way for oligosaccharides with different degree of polymerization (DP) was achieved by using solid acid degradation, which could give high yield and avoid solution neutralization process. Agaro-oligosaccharides with high purity were consequently obtained by activated carbon column isolation. Furthermore, the antioxidant and alpha-glucosidase inhibitory activity of three fractions were also investigated. The result indicated that 8% ethanol-eluted fraction showed highest activity against alpha-glucosidase with IC50 of 8.84 mg/mL, while 25% ethanol-eluted fraction possessed excellent antioxidant ability.
学科主题	Biotechnology & Applied Microbiology ; Food Science & Technology
语种	英语
WOS记录号	WOS:000227947900004
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3085]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Ningbo Univ, Key Lab Marine Biotechnol, Ningbo 315211, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Chen, HM,Zheng, L,Yan, XJ. The preparation and bioactivity research of agaro-oligosaccharides[J]. FOOD TECHNOLOGY AND BIOTECHNOLOGY,2005,43(1):29-36.
APA	Chen, HM,Zheng, L,&Yan, XJ.(2005).The preparation and bioactivity research of agaro-oligosaccharides.FOOD TECHNOLOGY AND BIOTECHNOLOGY,43(1),29-36.
MLA	Chen, HM,et al."The preparation and bioactivity research of agaro-oligosaccharides".FOOD TECHNOLOGY AND BIOTECHNOLOGY 43.1(2005):29-36.

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