Purification and characterization of the chitosanase from Aeromonas sp HG08

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	Purification and characterization of the chitosanase from Aeromonas sp HG08
	Sun, Yuying 2; Zhang, Jiquan 1; Wang, Shujun 2
刊名	AFRICAN JOURNAL OF BIOTECHNOLOGY
	2009-06-17
卷号	8 期号:12 页码:2830-2834
关键词	Aeromonas Sp. Chitosanase Purification Characterization
ISSN号	1684-5315
文献子类	Article
英文摘要	In this work, the characterization of a chitosanase-producing bacterium isolated from soil was reported and this strain was grouped under the genus Aeromonas by virtue of its morphological, physiological properties and 16S rDNA gene sequences. It is the first report that the genus Aeromonas could produce chitosanase. Aeromonas sp. HG08 could secrete the chitosanase ( named AsChi) with molecular weight of 70 kDa. The optimum pH and temperature of AsChi was 6.0 and 55 degrees C, respectively. The activity of AsChi was markedly enhanced by Mn(2+) and inhibited by Fe(3+), Cu(2+), Ag(+) and Hg(2+); additionally, the activity of AsChi was increased with the degree of deacetylation ( DDA) of chitosan. Through viscosimetric assay, AsChi probably hydrolyzed chitosan in an endo-type fashion.; In this work, the characterization of a chitosanase-producing bacterium isolated from soil was reported and this strain was grouped under the genus Aeromonas by virtue of its morphological, physiological properties and 16S rDNA gene sequences. It is the first report that the genus Aeromonas could produce chitosanase. Aeromonas sp. HG08 could secrete the chitosanase ( named AsChi) with molecular weight of 70 kDa. The optimum pH and temperature of AsChi was 6.0 and 55 degrees C, respectively. The activity of AsChi was markedly enhanced by Mn2+ and inhibited by Fe3+, Cu2+, Ag+ and Hg2+; additionally, the activity of AsChi was increased with the degree of deacetylation ( DDA) of chitosan. Through viscosimetric assay, AsChi probably hydrolyzed chitosan in an endo-type fashion.
学科主题	Biotechnology & Applied Microbiology
语种	英语
WOS记录号	WOS:000268831200028
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3021]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Huaihai Inst Technol, Coll Marine Sci, Lianyungang 222005, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yuying,Zhang, Jiquan,Wang, Shujun. Purification and characterization of the chitosanase from Aeromonas sp HG08[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(12):2830-2834.
APA	Sun, Yuying,Zhang, Jiquan,&Wang, Shujun.(2009).Purification and characterization of the chitosanase from Aeromonas sp HG08.AFRICAN JOURNAL OF BIOTECHNOLOGY,8(12),2830-2834.
MLA	Sun, Yuying,et al."Purification and characterization of the chitosanase from Aeromonas sp HG08".AFRICAN JOURNAL OF BIOTECHNOLOGY 8.12(2009):2830-2834.