Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01

doi:10.1007/s00343-009-9216-x

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	Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01
	Qiao Hongjin 1,2; Wang Guangce 1,3
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2009-11-01
卷号	27 期号:4 页码:762-768
关键词	Heterotrophic Microalgae Biodiesel Carbon Source Lipid
ISSN号	0254-4059
DOI	10.1007/s00343-009-9216-x
文献子类	Article
英文摘要	Heterotrophic culture of microalgae to develop methods of increasing biomass productivity and storage lipids has brought new insight to commercial biodiesel production. To understand the relationship between heterotrophy and lipid production, the effects of carbon sources on the growth and lipid accumulation of Chlorella sorokiniana GXNN01 was studied. The alga exhibited an increased growth rate in response to the addition of carbon sources, which reached the stationary phase after 48 h at 30A degrees C. In addition, glucose and NaAc had a significant effect on the lipid accumulation during the early-stationary phase. Specifically, the lipid content was 0.237 +/- 0.026 g g(-1) cell dry weight and 0.272 +/- 0.041 g L(-1) when glucose was used as the carbon source, whereas the lipid content reached 0.287 +/- 0.018 g g(-1) cell dry weight and 0.288 +/- 0.008 g L(-1) when NaAc was used as the carbon source. The neutral lipid content was found to first decrease and then increase over time during the growth phase. A glucose concentration of 20 mmol L(-1) gave the maximal lipid yield and the optimum harvest time was the early-stationary phase.; Heterotrophic culture of microalgae to develop methods of increasing biomass productivity and storage lipids has brought new insight to commercial biodiesel production. To understand the relationship between heterotrophy and lipid production, the effects of carbon sources on the growth and lipid accumulation of Chlorella sorokiniana GXNN01 was studied. The alga exhibited an increased growth rate in response to the addition of carbon sources, which reached the stationary phase after 48 h at 30A degrees C. In addition, glucose and NaAc had a significant effect on the lipid accumulation during the early-stationary phase. Specifically, the lipid content was 0.237 +/- 0.026 g g(-1) cell dry weight and 0.272 +/- 0.041 g L-1 when glucose was used as the carbon source, whereas the lipid content reached 0.287 +/- 0.018 g g(-1) cell dry weight and 0.288 +/- 0.008 g L-1 when NaAc was used as the carbon source. The neutral lipid content was found to first decrease and then increase over time during the growth phase. A glucose concentration of 20 mmol L-1 gave the maximal lipid yield and the optimum harvest time was the early-stationary phase.
学科主题	Limnology ; Oceanography
URL标识	查看原文
语种	英语
WOS记录号	WOS:000272422300010
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3003]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Tianjin Univ Sci & Technol, Tianjin 300222, Peoples R China
推荐引用方式 GB/T 7714	Qiao Hongjin,Wang Guangce. Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(4):762-768.
APA	Qiao Hongjin,&Wang Guangce.(2009).Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(4),762-768.
MLA	Qiao Hongjin,et al."Effect of carbon source on growth and lipid accumulation in Chlorella sorokiniana GXNN01".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.4(2009):762-768.