Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134

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	Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134
	Zhang, Wei-wei; Sun, Li; Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2007
卷号	73 期号:9 页码:2825-2831
关键词	Marine Bacterium Lactococcus-lactis Extracellular-agarase Glycosyl Hydrolases Secretion Signals Genomic Dna Purification Expression Classification Sequences
ISSN号	0099-2240
英文摘要	V134, a marine isolate of the Vibrio genus, was found to produce a new beta-agarase of the GH16 family. The relevant agarase gene agaV was cloned from V134 and conditionally expressed in Escherichia coli. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant agarase were around 40 degrees C and 7.0. AgaV was demonstrated to be useful in two aspects: first, as an agarolytic enzyme, the purified recombinant AgaV could be employed in the recovery of DNA from agarose gels; second, as a secretion protein, AgaV was explored at the genetic level and used as a reporter in the construction of a secretion signal trap which proved to be a simple and efficient molecular tool for the selection of genes encoding secretion proteins from both gram-positive and gram-negative bacteria.
学科主题	Biotechnology & Applied Microbiology ; Microbiology
URL标识	查看原文
公开日期	2010-11-18
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/1736]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wei-wei,Sun, Li,Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2007,73(9):2825-2831.
APA	Zhang, Wei-wei,Sun, Li,&Sun, L, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2007).Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,73(9),2825-2831.
MLA	Zhang, Wei-wei,et al."Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp strain V134".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 73.9(2007):2825-2831.