Genetic linkage map of bay scallop, Argopecten irradians irradians (Lamarck 1819)

doi:10.1111/j.1365-2109.2007.01683.x

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Genetic linkage map of bay scallop, Argopecten irradians irradians (Lamarck 1819)
	Wang, Lingling ; Song, Linsheng ; Zhang, Huan ; Gao, Qiang ; Guo, Ximing ; Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
刊名	AQUACULTURE RESEARCH
	2007-03-09
卷号	38 期号:4 页码:409-419
关键词	Linkage Map Aflp Ssr Scallop Argopecten Irradians Irradians
ISSN号	1355-557X
DOI	10.1111/j.1365-2109.2007.01683.x
文献子类	Article
英文摘要	The bay scallop (Argopecten irradians irradians Lamarck 1819) has become one of the most important aquaculture species in China. Genetic improvement of cultured bay scallop can benefit greatly from a better understanding of its genome. In this study, we developed amplified fragment length polymorphisms (AFLPs) and simple sequence repeat markers from expressed sequence tags (EST-SSRs) for linkage analysis in bay scallop. Segregation of 390 AFLP and eight SSR markers was analysed in a mapping population of 97 progeny. Of the AFLP markers analysed, 326 segregated in the expected 1:1 Mendelian ratio, while the remaining 74 (or 19.0%) showed significant deviation, with 33 (44.6%) being deficient in heterozygotes (A/a). Among the eight polymorphic EST-SSR loci, one marker (12.5%) was found skewing from its expected Mendelian ratios. Eighteen per cent of the markers segregating from female parent were distorted compared with 21% of the markers segregating from male parent. The female map included 147 markers in 17 linkage groups (LGs) and covered 1892.4 cM of the genome. In the male map, totally 146 AFLP and SSR markers were grouped in 18 LGs spanning 1937.1 cM. The average inter-marker spacing in female and male map was 12.9 and 13.3 cM respectively. The AFLP and SSR markers were distributed evenly throughout the genome except for a few large gaps over 20 cM. Although preliminary, the genetic maps presented here provide a starting point for the mapping of the bay scallop genome.; The bay scallop (Argopecten irradians irradians Lamarck 1819) has become one of the most important aquaculture species in China. Genetic improvement of cultured bay scallop can benefit greatly from a better understanding of its genome. In this study, we developed amplified fragment length polymorphisms (AFLPs) and simple sequence repeat markers from expressed sequence tags (EST-SSRs) for linkage analysis in bay scallop. Segregation of 390 AFLP and eight SSR markers was analysed in a mapping population of 97 progeny. Of the AFLP markers analysed, 326 segregated in the expected 1:1 Mendelian ratio, while the remaining 74 (or 19.0%) showed significant deviation, with 33 (44.6%) being deficient in heterozygotes (A/a). Among the eight polymorphic EST-SSR loci, one marker (12.5%) was found skewing from its expected Mendelian ratios. Eighteen per cent of the markers segregating from female parent were distorted compared with 21% of the markers segregating from male parent. The female map included 147 markers in 17 linkage groups (LGs) and covered 1892.4 cM of the genome. In the male map, totally 146 AFLP and SSR markers were grouped in 18 LGs spanning 1937.1 cM. The average inter-marker spacing in female and male map was 12.9 and 13.3 cM respectively. The AFLP and SSR markers were distributed evenly throughout the genome except for a few large gaps over 20 cM. Although preliminary, the genetic maps presented here provide a starting point for the mapping of the bay scallop genome.
学科主题	Fisheries
URL标识	查看原文
语种	英语
WOS记录号	WOS:000244716400009
公开日期	2010-11-18
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/1688]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Qingdao 266071, Peoples R China 3.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ USA
推荐引用方式 GB/T 7714	Wang, Lingling,Song, Linsheng,Zhang, Huan,et al. Genetic linkage map of bay scallop, Argopecten irradians irradians (Lamarck 1819)[J]. AQUACULTURE RESEARCH,2007,38(4):409-419.
APA	Wang, Lingling,Song, Linsheng,Zhang, Huan,Gao, Qiang,Guo, Ximing,&Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2007).Genetic linkage map of bay scallop, Argopecten irradians irradians (Lamarck 1819).AQUACULTURE RESEARCH,38(4),409-419.
MLA	Wang, Lingling,et al."Genetic linkage map of bay scallop, Argopecten irradians irradians (Lamarck 1819)".AQUACULTURE RESEARCH 38.4(2007):409-419.