Cloning and Comparative Studies of Seaweed Trehalose-6-Phosphate Synthase Genes | |
Wang, Guoliang2,3; Zhao, Ge2; Feng, Yanbin2; Xuan, Jinsong2; Sun, Jianwei2; Guo, Baotai4; Jiang, Guoyong4; Weng, Manli2; Yao, Jianting1; Wang, Bin2 | |
刊名 | MARINE DRUGS
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2010-07-01 | |
卷号 | 8期号:7页码:2065-2079 |
关键词 | Comparative Genomics Of Tps Genes Gene Cloning Race-pcr Seaweed Trehalose-6-phosphate Synthase Gene |
ISSN号 | 1660-3397 |
DOI | 10.3390/md8072065 |
文献子类 | Article |
英文摘要 | The full-length cDNA sequence (3219 base pairs) of the trehalose-6-phosphate synthase gene of Porphyra yezoensis (PyTPS) was isolated by RACE-PCR and deposited in GenBank (NCBI) with the accession number AY729671. PyTPS encodes a protein of 908 amino acids before a stop codon, and has a calculated molecular mass of 101,591 Daltons. The PyTPS protein consists of a TPS domain in the N-terminus and a putative TPP domain at the C-terminus. Homology alignment for PyTPS and the TPS proteins from bacteria, yeast and higher plants indicated that the most closely related sequences to PyTPS were those from higher plants (OsTPS and AtTPS5), whereas the most distant sequence to PyTPS was from bacteria (EcOtsAB). Based on the identified sequence of the PyTPS gene, PCR primers were designed and used to amplify the TPS genes from nine other seaweed species. Sequences of the nine obtained TPS genes were deposited in GenBank (NCBI). All 10 TPS genes encoded peptides of 908 amino acids and the sequences were highly conserved both in nucleotide composition (>94%) and in amino acid composition (>96%). Unlike the TPS genes from some other plants, there was no intron in any of the 10 isolated seaweed TPS genes.; The full-length cDNA sequence (3219 base pairs) of the trehalose-6-phosphate synthase gene of Porphyra yezoensis (PyTPS) was isolated by RACE-PCR and deposited in GenBank (NCBI) with the accession number AY729671. PyTPS encodes a protein of 908 amino acids before a stop codon, and has a calculated molecular mass of 101,591 Daltons. The PyTPS protein consists of a TPS domain in the N-terminus and a putative TPP domain at the C-terminus. Homology alignment for PyTPS and the TPS proteins from bacteria, yeast and higher plants indicated that the most closely related sequences to PyTPS were those from higher plants (OsTPS and AtTPS5), whereas the most distant sequence to PyTPS was from bacteria (EcOtsAB). Based on the identified sequence of the PyTPS gene, PCR primers were designed and used to amplify the TPS genes from nine other seaweed species. Sequences of the nine obtained TPS genes were deposited in GenBank (NCBI). All 10 TPS genes encoded peptides of 908 amino acids and the sequences were highly conserved both in nucleotide composition (>94%) and in amino acid composition (>96%). Unlike the TPS genes from some other plants, there was no intron in any of the 10 isolated seaweed TPS genes. |
学科主题 | Chemistry, Medicinal |
URL标识 | 查看原文 |
语种 | 英语 |
WOS记录号 | WOS:000280347100007 |
公开日期 | 2010-11-18 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/1682] ![]() |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
通讯作者 | Duan, DL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 4.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Guoliang,Zhao, Ge,Feng, Yanbin,et al. Cloning and Comparative Studies of Seaweed Trehalose-6-Phosphate Synthase Genes[J]. MARINE DRUGS,2010,8(7):2065-2079. |
APA | Wang, Guoliang.,Zhao, Ge.,Feng, Yanbin.,Xuan, Jinsong.,Sun, Jianwei.,...&Duan, DL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.(2010).Cloning and Comparative Studies of Seaweed Trehalose-6-Phosphate Synthase Genes.MARINE DRUGS,8(7),2065-2079. |
MLA | Wang, Guoliang,et al."Cloning and Comparative Studies of Seaweed Trehalose-6-Phosphate Synthase Genes".MARINE DRUGS 8.7(2010):2065-2079. |
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