GENETIC MAPPING OF THE LAMINARIA JAPONICA (LAMINARALES, PHAEOPHYTA) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS

doi:10.1111/j.1529-8817.2009.00729.x

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	GENETIC MAPPING OF THE LAMINARIA JAPONICA (LAMINARALES, PHAEOPHYTA) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS
	Liu, Fuli 1,2; Wang, Xiuliang 1; Liu, Jidong 1; Fu, Wandong 1,2; Duan, Delin 1; Yang, Yingxia 3; Wang, XL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
刊名	JOURNAL OF PHYCOLOGY
	2009-10-01
卷号	45 期号:5 页码:1228-1233
关键词	Amplified Fragment Length Polymorphism Genetic Improvement Genetic Linkage Map Laminaria Japonica Quantitative Trait Locus
ISSN号	0022-3646
DOI	10.1111/j.1529-8817.2009.00729.x
文献子类	Article
英文摘要	To establish a molecular-marker-assisted system of breeding and genetic study for Laminaria japonica Aresch., amplified fragment length polymorphism (AFLP) was used to construct a genetic linkage map of L. japonica featuring 230 progeny of F(2) cross population. Eighteen primer combinations produced 370 polymorphic loci and 215 polymorphic loci segregated in a 3:1 Mendelian segregation ratio (P <= 0.05). Of the 215 segregated loci, 142 were ordered into 27 linkage groups. The length of the linkage groups ranged from 6.7 to 90.3 centimorgans (cM) with an average length of 49.6 cM, and the total length was 1,085.8 cM, which covered 68.4% of the estimated 1,586.9 cM genome. The number of mapped markers on each linkage group ranged from 2 to 12, averaging 5.3 markers per group. The average density of the markers was 1 per 9.4 cM. Based on the marker density and the resolution of the map, the constructed linkage map can satisfy the need for quantitative trait locus (QTL) location and molecular-marker-assisted breeding for Laminaria.; To establish a molecular-marker-assisted system of breeding and genetic study for Laminaria japonica Aresch., amplified fragment length polymorphism (AFLP) was used to construct a genetic linkage map of L. japonica featuring 230 progeny of F-2 cross population. Eighteen primer combinations produced 370 polymorphic loci and 215 polymorphic loci segregated in a 3:1 Mendelian segregation ratio (P <= 0.05). Of the 215 segregated loci, 142 were ordered into 27 linkage groups. The length of the linkage groups ranged from 6.7 to 90.3 centimorgans (cM) with an average length of 49.6 cM, and the total length was 1,085.8 cM, which covered 68.4% of the estimated 1,586.9 cM genome. The number of mapped markers on each linkage group ranged from 2 to 12, averaging 5.3 markers per group. The average density of the markers was 1 per 9.4 cM. Based on the marker density and the resolution of the map, the constructed linkage map can satisfy the need for quantitative trait locus (QTL) location and molecular-marker-assisted breeding for Laminaria.
学科主题	Plant Sciences ; Marine & Freshwater Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000270901800024
公开日期	2010-11-18
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/1646]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, XL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100059, Peoples R China 3.Ludong Univ, Yantai 264025, Peoples R China
推荐引用方式 GB/T 7714	Liu, Fuli,Wang, Xiuliang,Liu, Jidong,et al. GENETIC MAPPING OF THE LAMINARIA JAPONICA (LAMINARALES, PHAEOPHYTA) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS[J]. JOURNAL OF PHYCOLOGY,2009,45(5):1228-1233.
APA	Liu, Fuli.,Wang, Xiuliang.,Liu, Jidong.,Fu, Wandong.,Duan, Delin.,...&Wang, XL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.(2009).GENETIC MAPPING OF THE LAMINARIA JAPONICA (LAMINARALES, PHAEOPHYTA) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS.JOURNAL OF PHYCOLOGY,45(5),1228-1233.
MLA	Liu, Fuli,et al."GENETIC MAPPING OF THE LAMINARIA JAPONICA (LAMINARALES, PHAEOPHYTA) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS".JOURNAL OF PHYCOLOGY 45.5(2009):1228-1233.