Characterization of 30 microsatellite markers in distylous Primula sinolisteri (Primulaceae) using HiSeq sequencing

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	Characterization of 30 microsatellite markers in distylous Primula sinolisteri (Primulaceae) using HiSeq sequencing
	Wang, Xinjia 1,2; Zhong, Li 1,2; Wu, Zhikun 3; Sun, Huaying 1; Wang, Hong1 ; Li, Dezhu4 ; Barrett, Spencer C. H.5; Zhou, Wei 4
刊名	APPLICATIONS IN PLANT SCIENCES
	2019
卷号	7 期号:1 页码:5
关键词	distyly HiSeq homostyly microsatellites Primula sinolisteri Primulaceae
ISSN号	2168-0450
英文摘要	Premise of the Study Microsatellite markers were developed for Primula sinolisteri, a perennial distylous herb belonging to section Obconicolisteri (Primulaceae), to facilitate future investigations of the population genetics and mating patterns of populations in this species. Methods and Results We developed 30 microsatellite markers for P. sinolisteri using HiSeq X-Ten sequencing and measured polymorphism and genetic diversity in a sample of 36 individuals from three natural populations. The markers displayed relatively high polymorphism, with the number of observed alleles per locus ranging from one to 19 (mean = 4.42). The observed and expected heterozygosity ranged from 0-1.000 and 0.083-0.882, respectively. Twenty-nine of the loci were also successfully amplified in homostylous P. sinolisteri var. aspera. Conclusions The microsatellite markers we have identified in P. sinolisteri provide powerful tools for investigating patterns of population genetic diversity and the evolutionary relationships between heterostyly and homostyly in this species.
资助项目	National Natural Science Foundation of China[31570384] ; National Natural Science Foundation of China[31770417] ; Joint Fund of National Natural Science Foundation of China-Yunnan Province[U1502261] ; Light of West China Program of the Chinese Academic of Sciences
语种	英语
资助机构	National Natural Science Foundation of China ; Joint Fund of National Natural Science Foundation of China-Yunnan Province ; Light of West China Program of the Chinese Academic of Sciences
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/64238]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者	Zhou, Wei
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Guiyang Univ Tradit Chinese Med, Dept Pharm, Guiyang 550002, Guizhou, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China 5.Univ Toronto, Dept Ecol & Evolutionary Biol, Toronto, ON M5S 3B2, Canada
推荐引用方式 GB/T 7714	Wang, Xinjia,Zhong, Li,Wu, Zhikun,et al. Characterization of 30 microsatellite markers in distylous Primula sinolisteri (Primulaceae) using HiSeq sequencing[J]. APPLICATIONS IN PLANT SCIENCES,2019,7(1):5.
APA	Wang, Xinjia.,Zhong, Li.,Wu, Zhikun.,Sun, Huaying.,Wang, Hong.,...&Zhou, Wei.(2019).Characterization of 30 microsatellite markers in distylous Primula sinolisteri (Primulaceae) using HiSeq sequencing.APPLICATIONS IN PLANT SCIENCES,7(1),5.
MLA	Wang, Xinjia,et al."Characterization of 30 microsatellite markers in distylous Primula sinolisteri (Primulaceae) using HiSeq sequencing".APPLICATIONS IN PLANT SCIENCES 7.1(2019):5.