Effect of PA-MSHA vaccine on plasma phospholipids metabolic profiling and the ratio of Th2/Th1 cells within immune organ of mouse IgA nephropathy

	Effect of PA-MSHA vaccine on plasma phospholipids metabolic profiling and the ratio of Th2/Th1 cells within immune organ of mouse IgA nephropathy
	Jia, Lewen ; Wang, Chang ; Kong, Hongwei ; Yang, Jun ; Li, Fanglou ; Lv, Shen ; Xu, Guowang
刊名	journal of pharmaceutical and biomedical analysis
	2007-01-17
卷号	43 期号:2 页码:646-654
关键词	IgA nephropathy phospholipid PA-MSHA vaccine Th-2/Th-1 high performance liquid chromatography metabolic profiling
通讯作者	许国旺
产权排序	1;1
英文摘要	phospholipids as a class of important constituents in the biomembranes have been paid increasing attention in many fields. iga nephropathy is now generally known to be the most common form of primary glomerulonephritis in the world. however, phospholipids metabolism in iga nephropathy was not clear. until recently, there was no effective treatment available for patients with iga nephropathy. in this paper, effect of pa-msha vaccine on plasma phospholipid metabolic profile of mouse iga nephropathy was investigated using high performance liquid chromatography/mass spectrometry (hplums) and principal components analysis (pca). female balb/c mice were divided into four groups: model group, control group, pa-msha treatment group and medicine control group (dipyridamole + common threewingnut root). the experimental iga nephropathy model was established by the immunity combination method of oral bsa and injection of seb. it was found that combination of lc/ms technology with pca can be successfully applied to phospholipids profile analysis, clearly classify the model group and normal group, and pa-msha treatment group is closer to the normal control group than medicine control group. the result showed that th-2/th-1 (=cd4+cd30+/cd4+cd30-) of the model group is 20.70 +/- 3.57, which is significantly higher than that of the control group (1.34 +/- 0.14) (p < 0.001). the th-2/th-1 ratio of the pa-msha treatment group and the medicine control group are lower than that of the model group (p < 0.01). it is suggested that mouse iga nephropathy has the phospholipids metabolic abnormality, pa-msha vaccine cannot only regulate the abnormal phospholipids metabolism mouse with the iga nephropathy, but also correct the over unbalance of th-2/th-1 proportion. (c) 2006 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences ; life sciences & biomedicine
类目[WOS]	chemistry, analytical ; pharmacology & pharmacy
研究领域[WOS]	chemistry ; pharmacology & pharmacy
关键词[WOS]	trap mass-spectrometry ; human blood ; metabonomics ; identification ; urine ; spectroscopy ; recognition ; severity ; acid ; gene
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000243802700035
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/98775]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 1, Dept Nephrol, Dalian 116011, Peoples R China 3.Dalian Med Univ, Affiliated Hosp 1, Expt Ctr, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Jia, Lewen,Wang, Chang,Kong, Hongwei,et al. Effect of PA-MSHA vaccine on plasma phospholipids metabolic profiling and the ratio of Th2/Th1 cells within immune organ of mouse IgA nephropathy[J]. journal of pharmaceutical and biomedical analysis,2007,43(2):646-654.
APA	Jia, Lewen.,Wang, Chang.,Kong, Hongwei.,Yang, Jun.,Li, Fanglou.,...&Xu, Guowang.(2007).Effect of PA-MSHA vaccine on plasma phospholipids metabolic profiling and the ratio of Th2/Th1 cells within immune organ of mouse IgA nephropathy.journal of pharmaceutical and biomedical analysis,43(2),646-654.
MLA	Jia, Lewen,et al."Effect of PA-MSHA vaccine on plasma phospholipids metabolic profiling and the ratio of Th2/Th1 cells within immune organ of mouse IgA nephropathy".journal of pharmaceutical and biomedical analysis 43.2(2007):646-654.