Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field

	Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field
	Cao, Xupeng ; Fu, Wantao ; Yu, Xingju ; Zhang, Wei
刊名	cell and tissue research
	2007-09-01
卷号	329 期号:3 页码:595-608
关键词	silica spicule silicatein spiculogenesis archaeocyte-dominant cell populations primmorph culture sponge Hymeniacidon perlevis (Porifera)
通讯作者	张卫
产权排序	1;1
英文摘要	to characterize the formation of silica spicules, the dynamics of spiculogenesis of an intertidal marine sponge hymeniacidon perlevis (montagu 1818) (porifera: demospongiae) were investigated by measuring the gene expression of silicatein (the enzyme responsible for spicule silicification) and the dimensional changes of spicules during the developmental process of individual sponges and in cell cultures of primmorphs of archaeocyte-dominant cell populations. the different developmental stages of spicules were documented by time-lapse microscopy and observed by transmission electron microscopy during a 1-month culture period. during its annual life cycle, h. perlevis has four different developmental stages: dormancy, resuscitation, bloom, and decline. field-grown individual sponge samples at different stages were collected over 7 months (march to september 2005). the dimensions of the silica spicules from these samples were microscopically measured and statistically analyzed. this analysis and the material properties of the spicules allowed them to be classified into four groups representing the different developmental stages of spiculogenesis. silicatein expression in the bloom stage was more than 100 times higher than that in the other stages and was correlated with the spicule developmental stage. the trend of spicule formation in field-grown sponges was consistent with the trend in cell culture. a new parameter, the maturation degree (md) of spicules (defined as the ratio of actual to theoretical silica deposition of mature spicules), was introduced to quantify spicule development. silica spiculogenesis during h. perlevis development was delineated by comparing md and silicatein expression.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	cell biology
研究领域[WOS]	cell biology
关键词[WOS]	demosponge suberites-domuncula ; silicatein filaments ; ephydatia-muelleri ; siliceous spicules ; primmorphs ; spiculogenesis ; proliferation ; sclerocytes ; lectin ; larvae
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000249128400016
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/98715]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 10089, Peoples R China 3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Mol Bioproc & Bioprod Lab, Adelaide, SA 5042, Australia
推荐引用方式 GB/T 7714	Cao, Xupeng,Fu, Wantao,Yu, Xingju,et al. Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field[J]. cell and tissue research,2007,329(3):595-608.
APA	Cao, Xupeng,Fu, Wantao,Yu, Xingju,&Zhang, Wei.(2007).Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field.cell and tissue research,329(3),595-608.
MLA	Cao, Xupeng,et al."Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field".cell and tissue research 329.3(2007):595-608.