Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis

	Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis
	Shen, Zheng ; Liu, Xiaojun ; Zhou, Xiaomian ; Liang, Aiye ; Wu, Dapeng ; Yu, Linfen ; Dai, Zhongpeng ; Qin, Jianhua ; Lin, Bingcheng
刊名	journal of separation science
	2007-07-01
卷号	30 期号:10 页码:1544-1548
关键词	capillary array electrophoresis DNA interaction microfluidic chip netropsin
通讯作者	林炳承
产权排序	1;1
英文摘要	microfabricated capillary array electrophoresis (mu-cae) was applied to study the interaction between minor groove binder netropsin and a non-selfcomplementary 12 mer double stranded oligodeoxynucleotide: d(cccctataccgc) center dot d(gcggtatagggg). esi-ms was used to provide an independent verification of the microchip electrophoresis derived data. simultaneous parallel quantitative assay of multiple samples was performed in a single run (< 50 s) on the self-developed mu-cae device. the binding constant and stoichiometry calculated from scatchard plot were (2.88 +/- 0.23) x 10(5) m-1 and 1:1, respectively. the values showed a good quantitative agreement with the results determined by esi-ms and those using other methods reported in the literature.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	ionization mass-spectrometry ; drug-dna interactions ; zone-electrophoresis ; analysis systems ; affinity ; sequence ; chip ; selectivity ; complexes
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000248240000018
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/98701]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Guangzhou Med Coll, Dept Clin Lab, Guangzhou Municipal Peoples Hosp 1, Guangzhou, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Shen, Zheng,Liu, Xiaojun,Zhou, Xiaomian,et al. Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis[J]. journal of separation science,2007,30(10):1544-1548.
APA	Shen, Zheng.,Liu, Xiaojun.,Zhou, Xiaomian.,Liang, Aiye.,Wu, Dapeng.,...&Lin, Bingcheng.(2007).Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis.journal of separation science,30(10),1544-1548.
MLA	Shen, Zheng,et al."Quantitative evaluation of the interaction between netropsin and double stranded oligodeoxynucleotides by microfabricated capillary array electrophoresis".journal of separation science 30.10(2007):1544-1548.