Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device

	Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device
	Ye, Nannan; Qin, Jianhua; Shi, Weiwei; Lin, Bingcheng
刊名	electrophoresis
	2007-04-01
卷号	28 期号:7 页码:1146-1153
关键词	apoptosis doxorubicin human hepatocellular carcinoma cells microfluidic device
通讯作者	林炳承
产权排序	1;1
英文摘要	apoptosis has now established its importance in numerous areas of biology and is recently receiving great attention as an important topic related to the development of diseases. in this work, an integrated microfluidic device was developed to characterize doxorubicin-induced apoptosis in human hepatocellular carcinoma (hepg2) cells. a continuous concentration gradient of stimulator (doxorubicin) was generated in the upstream network and used to perfuse downstream cultured hepg2 cells. the appropriate fluorescent dyes were introduced into cells from the inlets connected to the cell culture chambers, allowing one to distinguish apoptotic cells from nonapoptotic or necrotic cells. the resultant fluorescence of cellular population was monitored and quantified with single-cell resolution to infer the apoptosis process being studied. the feasibility of studying apoptosis was demonstrated by measuring several apoptotic events, including morphological alterations, plasma membrane phosphatidylserine externalization, and mitochondrial membrane potential collapse. this microfluidic device, integrating the cell culture, stimulation, staining, and washing steps into a single device, can simultaneously generate a number of experimental conditions and investigate multiple parameters relating stimulation to apoptosis. it offers a unique platform to characterize various cellular responses in a high-throughput fashion, which is otherwise impossible with conventional methods.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	total analysis systems ; differentiation ; generation ; gradients ; hallmarks ; death ; flow
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000245682000015
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/98693]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Ye, Nannan,Qin, Jianhua,Shi, Weiwei,et al. Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device[J]. electrophoresis,2007,28(7):1146-1153.
APA	Ye, Nannan,Qin, Jianhua,Shi, Weiwei,&Lin, Bingcheng.(2007).Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device.electrophoresis,28(7),1146-1153.
MLA	Ye, Nannan,et al."Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device".electrophoresis 28.7(2007):1146-1153.