Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome analysis by using a strong cation exchange trap column

	Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome analysis by using a strong cation exchange trap column
	Jiang, Xiaogang ; Feng, Shun ; Tian, Ruijun ; Han, Guanghui ; Jiang, Xinning ; Ye, Mingliang ; Zou, Hanfa
刊名	proteomics
	2007-02-01
卷号	7 期号:4 页码:528-539
关键词	automation mu LC-MS/MS sample injection SCX trap column
ISSN号	1615-9853
通讯作者	邹汉法
产权排序	1;1
英文摘要	an approach was developed to automate sample introduction for nanoflow lc-ms/ms (mu lc-ms/ ms) analysis using a strong cation exchange (scx) trap column. the system consisted of a 100 mu m id x 2 cm scx trap column and a 75 mu m id x 12 cm c18 rp analytical column. during the sample loading step, the flow passing through the scx trap column was directed to waste for loading a large volume of sample at high flow rate. then the peptides bound on the scx trap column were eluted onto the rp analytical column by a high salt buffer followed by rp chromatographic separation of the peptides at nanoliter flow rate. it was observed that higher performance of separation could be achieved with the system using scx trap column than with the system using c18 trap column. the high proteomic coverage using this approach was demonstrated in the analysis of tryptic digest of bsa and yeast cell lysate. in addition, this system was also applied to two-dimensional separation of tryptic digest of human hepatocellular carcinoma cell line smmc-7721 for large scale proteome analysis. this system was fully automated and required minimum changes on current mu lc-ms/ms system. this system represented a promising platform for routine proteome analysis.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods ; biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	multidimensional chromatography ; identification technology ; polyacrylamide-gel ; yeast proteome ; ms system ; electrospray ; ionization ; capillary ; proteins ; peptide
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000244807500006
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/98477]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Suzhou Univ, Sch Med, Suzhou 215006, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Xiaogang,Feng, Shun,Tian, Ruijun,et al. Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome analysis by using a strong cation exchange trap column[J]. proteomics,2007,7(4):528-539.
APA	Jiang, Xiaogang.,Feng, Shun.,Tian, Ruijun.,Han, Guanghui.,Jiang, Xinning.,...&Zou, Hanfa.(2007).Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome analysis by using a strong cation exchange trap column.proteomics,7(4),528-539.
MLA	Jiang, Xiaogang,et al."Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome analysis by using a strong cation exchange trap column".proteomics 7.4(2007):528-539.