Preparation and evaluation of a molecularly imprinted polymer derivatized silica monolithic column for capillary electrochromatography and capillary liquid chromatography

	Preparation and evaluation of a molecularly imprinted polymer derivatized silica monolithic column for capillary electrochromatography and capillary liquid chromatography
	Ou, Junjie ; Li, Xin ; Feng, Shun ; Dong, Jing ; Dong, Xiaoli ; Kong, Liang ; Ye, Mingliang ; Zou, Hanfa
刊名	analytical chemistry
	2007-01-15
卷号	79 期号:2 页码:639-646
ISSN号	0003-2700
通讯作者	邹汉法
产权排序	1;1
英文摘要	a method for preparation of molecularly imprinted polymer (mip) derivatized onto the surface of a monolithic silica capillary column was successfully developed. the vinyl groups were first introduced onto the silica monolith by immobilization of gamma-methacryloxypropyltrimethoxysilane. then the mip coating was copolymerized and anchored onto the surface of the silica monolith. acetonitrile was selected as porogen (solvent). the other preparation conditions, such as monomer concentration, temperature, and time of polymerization, were systematically studied. the obtained mip-derivatized silica monolith using l-tetrahydropalmatine (l-thp) and (5s,11s)-(-)-troger's base (s-tb) as the imprinted template, respectively, was characterized in terms of the retention behavior of thiourea and toluene. under the optimized cec conditions, baseline enantioseparations of thp and tb were achieved in 4 min though the effective length of the columns was 8.5 cm. the result indicates that enough recognition sites were on the surface of silica monolith, resulting in strong recognition ability. compared with a mip organic monolith, the mip-derivatized silica monolith exhibits better column efficiency and stability in cec. additionally, the comparison of these two kinds of monolithic columns was performed by capillary liquid chromatography. the separation on mip-derivatized silica monolith was superior to that on the organic monolith.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	stationary phases ; enantiomer separation ; analytical-chemistry ; recognition ; selectivity ; pharmaceuticals ; diastereomers ; technology ; coatings
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000243415800031
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/98443]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Zhejiang Univ, Dept Chem, Hangzhou 310028, Peoples R China
推荐引用方式 GB/T 7714	Ou, Junjie,Li, Xin,Feng, Shun,et al. Preparation and evaluation of a molecularly imprinted polymer derivatized silica monolithic column for capillary electrochromatography and capillary liquid chromatography[J]. analytical chemistry,2007,79(2):639-646.
APA	Ou, Junjie.,Li, Xin.,Feng, Shun.,Dong, Jing.,Dong, Xiaoli.,...&Zou, Hanfa.(2007).Preparation and evaluation of a molecularly imprinted polymer derivatized silica monolithic column for capillary electrochromatography and capillary liquid chromatography.analytical chemistry,79(2),639-646.
MLA	Ou, Junjie,et al."Preparation and evaluation of a molecularly imprinted polymer derivatized silica monolithic column for capillary electrochromatography and capillary liquid chromatography".analytical chemistry 79.2(2007):639-646.