A new terbium(III) chelate as an efficient singlet oxygen fluorescence probe

	A new terbium(III) chelate as an efficient singlet oxygen fluorescence probe
	Tan, MQ; Song, B; Wang, GL; Yuan, JL
刊名	free radical biology and medicine
	2006-05-01
卷号	40 期号:9 页码:1644-1653
关键词	terbium fluorescence probe singlet oxygen time-resolved fluorescence detection free radicals
ISSN号	0891-5849
通讯作者	袁景利
产权排序	1;1
英文摘要	a terbium(iii) chelate fluorescence probe for detection of singlet oxygen (102) in aqueous media, nnn',n-[2,6-bis(3'-aminomethyl-l'pyrazolyi)-4-(9"-anthryl)pyridine] tetrakis (acetate)-tb3+ (pata-tb3+), was designed and synthesized. the new chelate is highly water soluble, is almost nonfluorescent, and can specifically react with o-1(2) to yield a strongly fluorescent chelate, the endoperoxide of pata-tb3+, accompanied by a remarkable increase in the fluorescence quantum yield from 0.46 to 10.5%. the long fluorescence lifetime of the endoperoxide (2.76 ms) allows the probe to be used favorably for time-resolved fluorescence detection of o-1(2). the studies of fluorescence property and reaction specificity indicate that the new probe is highly sensitive and selective for o-1(2). the probe was used for quantitative detection of o-1(2) generated from a moo42--h2o2 system to give a detection limit of 10.8 nm. in addition, the good applicability of the probe was demonstrated by the real-time monitoring of the kinetic process of o-1(2) generation in a horseradish peroxidase-catalyzed oxidation system of indole-3-acetic acid in a weakly acidic buffer. (c) 2006 elsevier inc. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; endocrinology & metabolism
研究领域[WOS]	biochemistry & molecular biology ; endocrinology & metabolism
关键词[WOS]	peroxidase-catalyzed oxidation ; hydrogen-peroxide ; molecular-oxygen ; bioanalytical assays ; photodynamic therapy ; rational design ; murine tumor ; generation ; sensitizer ; identification
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000237433900016
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/97921]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dept Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Tan, MQ,Song, B,Wang, GL,et al. A new terbium(III) chelate as an efficient singlet oxygen fluorescence probe[J]. free radical biology and medicine,2006,40(9):1644-1653.
APA	Tan, MQ,Song, B,Wang, GL,&Yuan, JL.(2006).A new terbium(III) chelate as an efficient singlet oxygen fluorescence probe.free radical biology and medicine,40(9),1644-1653.
MLA	Tan, MQ,et al."A new terbium(III) chelate as an efficient singlet oxygen fluorescence probe".free radical biology and medicine 40.9(2006):1644-1653.