A new terbium(III) chelate as an efficient singlet oxygen fluorescence probe | |
Tan, MQ; Song, B; Wang, GL; Yuan, JL | |
刊名 | free radical biology and medicine
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2006-05-01 | |
卷号 | 40期号:9页码:1644-1653 |
关键词 | terbium fluorescence probe singlet oxygen time-resolved fluorescence detection free radicals |
ISSN号 | 0891-5849 |
通讯作者 | 袁景利 |
产权排序 | 1;1 |
英文摘要 | a terbium(iii) chelate fluorescence probe for detection of singlet oxygen (102) in aqueous media, nnn',n-[2,6-bis(3'-aminomethyl-l'pyrazolyi)-4-(9"-anthryl)pyridine] tetrakis (acetate)-tb3+ (pata-tb3+), was designed and synthesized. the new chelate is highly water soluble, is almost nonfluorescent, and can specifically react with o-1(2) to yield a strongly fluorescent chelate, the endoperoxide of pata-tb3+, accompanied by a remarkable increase in the fluorescence quantum yield from 0.46 to 10.5%. the long fluorescence lifetime of the endoperoxide (2.76 ms) allows the probe to be used favorably for time-resolved fluorescence detection of o-1(2). the studies of fluorescence property and reaction specificity indicate that the new probe is highly sensitive and selective for o-1(2). the probe was used for quantitative detection of o-1(2) generated from a moo42--h2o2 system to give a detection limit of 10.8 nm. in addition, the good applicability of the probe was demonstrated by the real-time monitoring of the kinetic process of o-1(2) generation in a horseradish peroxidase-catalyzed oxidation system of indole-3-acetic acid in a weakly acidic buffer. (c) 2006 elsevier inc. all rights reserved. |
WOS标题词 | science & technology ; life sciences & biomedicine |
类目[WOS] | biochemistry & molecular biology ; endocrinology & metabolism |
研究领域[WOS] | biochemistry & molecular biology ; endocrinology & metabolism |
关键词[WOS] | peroxidase-catalyzed oxidation ; hydrogen-peroxide ; molecular-oxygen ; bioanalytical assays ; photodynamic therapy ; rational design ; murine tumor ; generation ; sensitizer ; identification |
收录类别 | SCI |
原文出处 | true |
语种 | 英语 |
WOS记录号 | WOS:000237433900016 |
公开日期 | 2010-11-30 |
内容类型 | 期刊论文 |
源URL | [http://159.226.238.44/handle/321008/97921] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dept Analyt Chem, Dalian 116023, Peoples R China |
推荐引用方式 GB/T 7714 | Tan, MQ,Song, B,Wang, GL,et al. A new terbium(III) chelate as an efficient singlet oxygen fluorescence probe[J]. free radical biology and medicine,2006,40(9):1644-1653. |
APA | Tan, MQ,Song, B,Wang, GL,&Yuan, JL.(2006).A new terbium(III) chelate as an efficient singlet oxygen fluorescence probe.free radical biology and medicine,40(9),1644-1653. |
MLA | Tan, MQ,et al."A new terbium(III) chelate as an efficient singlet oxygen fluorescence probe".free radical biology and medicine 40.9(2006):1644-1653. |
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