New luminescent europium(III) chelates for DNA labeling

	New luminescent europium(III) chelates for DNA labeling
	Nishioka, T ; Yuan, JL ; Yamamoto, Y ; Sumitomo, K ; Wang, Z ; Hashino, K ; Hosoya, C ; Ikawa, K ; Wang, GL ; Matsumoto, K
刊名	inorganic chemistry
	2006-05-15
卷号	45 期号:10 页码:4088-4096
ISSN号	0020-1669
通讯作者	kazuko matsumoto
产权排序	3;2
英文摘要	the new europium(iii) chelate {2,2', 2", 2"'-{[4'-(aminobiphenyl-4-yl)-2,2': 6',2"-terpyridine-6,6"-diyl]bis(methylenenitrilo)}-tetrakis( acetato)} europium(iii) (atbta-eu3+) and its 4,6-dichloro-1,3,5-triazinyl and succinimidyl derivatives ( dtbta and nhs-atbta, respectively) were synthesized and characterized. both labeling complexes dtbta-eu3+ and nhs-atbta-eu3+ are luminescent. especially dtbta-eu3+ is strongly luminescent, with a luminescence quantum yield of 9.1%, molar extinction coefficient of 3.1 x 104 cm(-1) m-1 ( 335 nm), and luminescence lifetime of 1.02 ms. the excitation and emission maximum wavelengths of dtbta-eu3+ are 335 and 616 nm, respectively. the complex is very stable in aqueous buffers, with a conditional formation constant log kdtbta-eu of 25.0 at ph 8, and can be conjugated to dna and proteins. the chelates are also highly resistant to thermal decomposition, photodegradation, and ozone oxidation. these properties prove that dtbta-eu3+ is suitable as a luminescence label in dna assays.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, inorganic & nuclear
研究领域[WOS]	chemistry
关键词[WOS]	time-resolved fluoroimmunoassay ; state energy-level ; lanthanide complexes ; fluorescent chelate ; bioanalytical assays ; aqueous-solution ; derivatives ; binding ; agents ; 2,2'/6',2''-terpyridine
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000237593100038
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/97919]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan 2.Waseda Univ, Adv Res Inst Sci & Engn, Shinjuku Ku, Tokyo 1698555, Japan 3.Chinese Acad Sci, Dept Analyt Chem, Dalian Inst Chem Phys, Dalian 116012, Peoples R China 4.Japan Sci & Technol Agcy, Kawaguchi, Japan
推荐引用方式 GB/T 7714	Nishioka, T,Yuan, JL,Yamamoto, Y,et al. New luminescent europium(III) chelates for DNA labeling[J]. inorganic chemistry,2006,45(10):4088-4096.
APA	Nishioka, T.,Yuan, JL.,Yamamoto, Y.,Sumitomo, K.,Wang, Z.,...&Matsumoto, K.(2006).New luminescent europium(III) chelates for DNA labeling.inorganic chemistry,45(10),4088-4096.
MLA	Nishioka, T,et al."New luminescent europium(III) chelates for DNA labeling".inorganic chemistry 45.10(2006):4088-4096.