New luminescent europium(III) chelates for DNA labeling | |
Nishioka, T; Yuan, JL; Yamamoto, Y; Sumitomo, K; Wang, Z; Hashino, K; Hosoya, C; Ikawa, K; Wang, GL; Matsumoto, K | |
刊名 | inorganic chemistry
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2006-05-15 | |
卷号 | 45期号:10页码:4088-4096 |
ISSN号 | 0020-1669 |
通讯作者 | kazuko matsumoto |
产权排序 | 3;2 |
英文摘要 | the new europium(iii) chelate {2,2', 2", 2"'-{[4'-(aminobiphenyl-4-yl)-2,2': 6',2"-terpyridine-6,6"-diyl]bis(methylenenitrilo)}-tetrakis( acetato)} europium(iii) (atbta-eu3+) and its 4,6-dichloro-1,3,5-triazinyl and succinimidyl derivatives ( dtbta and nhs-atbta, respectively) were synthesized and characterized. both labeling complexes dtbta-eu3+ and nhs-atbta-eu3+ are luminescent. especially dtbta-eu3+ is strongly luminescent, with a luminescence quantum yield of 9.1%, molar extinction coefficient of 3.1 x 104 cm(-1) m-1 ( 335 nm), and luminescence lifetime of 1.02 ms. the excitation and emission maximum wavelengths of dtbta-eu3+ are 335 and 616 nm, respectively. the complex is very stable in aqueous buffers, with a conditional formation constant log kdtbta-eu of 25.0 at ph 8, and can be conjugated to dna and proteins. the chelates are also highly resistant to thermal decomposition, photodegradation, and ozone oxidation. these properties prove that dtbta-eu3+ is suitable as a luminescence label in dna assays. |
WOS标题词 | science & technology ; physical sciences |
类目[WOS] | chemistry, inorganic & nuclear |
研究领域[WOS] | chemistry |
关键词[WOS] | time-resolved fluoroimmunoassay ; state energy-level ; lanthanide complexes ; fluorescent chelate ; bioanalytical assays ; aqueous-solution ; derivatives ; binding ; agents ; 2,2'/6',2''-terpyridine |
收录类别 | SCI |
原文出处 | true |
语种 | 英语 |
WOS记录号 | WOS:000237593100038 |
公开日期 | 2010-11-30 |
内容类型 | 期刊论文 |
源URL | [http://159.226.238.44/handle/321008/97919] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Waseda Univ, Dept Chem, Shinjuku Ku, Tokyo 1698555, Japan 2.Waseda Univ, Adv Res Inst Sci & Engn, Shinjuku Ku, Tokyo 1698555, Japan 3.Chinese Acad Sci, Dept Analyt Chem, Dalian Inst Chem Phys, Dalian 116012, Peoples R China 4.Japan Sci & Technol Agcy, Kawaguchi, Japan |
推荐引用方式 GB/T 7714 | Nishioka, T,Yuan, JL,Yamamoto, Y,et al. New luminescent europium(III) chelates for DNA labeling[J]. inorganic chemistry,2006,45(10):4088-4096. |
APA | Nishioka, T.,Yuan, JL.,Yamamoto, Y.,Sumitomo, K.,Wang, Z.,...&Matsumoto, K.(2006).New luminescent europium(III) chelates for DNA labeling.inorganic chemistry,45(10),4088-4096. |
MLA | Nishioka, T,et al."New luminescent europium(III) chelates for DNA labeling".inorganic chemistry 45.10(2006):4088-4096. |
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