Preparation and application of monolithic columns with narrow immobilized pH gradients

	Preparation and application of monolithic columns with narrow immobilized pH gradients
	Zhu, Guijie ; Yang, Chun ; Zhang, Lihua ; Liang, Zhen ; Zhang, Weibing ; Zhang, Yukui
刊名	talanta
	2006-08-15
卷号	70 期号:1 页码:2-6
关键词	monolithic immobilized pH gradient column preparation narrow pH gradient
通讯作者	张丽华
产权排序	1;1
英文摘要	monolith with immobilized ph gradient (m-ipg) is a novel separation matrix for amphoteric substances, such as peptides and proteins. to improve the properties of the newly designed column, efforts were made to optimize the preparation procedure, including the concentrations of the monomers, glycidyl methacrylate (gma) and ethylene glycol dimethacrylate (edma), as well as the kinds of porogens, which led to a monolith with improved permeability, uniformity and continuity. in addition, different diamines, including ethylenediamine, 1,3-diaminopropane, 1,4-diaminobutane and 1,5-diaminopentane, were subjected to aminate the polymer, and the last two exhibited excellent reactivity with epoxy on the polymer surface, which could obviously make the immobilization of ph gradient facilitated. under the optimal conditions, a simple method to prepare m-ipg column with narrow ph gradient was developed with commercial carrier ampholytes (cas) solution. such columns were applied into the analysis of proteins and peptides, and showed the improved resolution compared to the traditional one with a wide ph distribution. (c) 2005 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	capillary electrochromatography ; carrier ampholytes ; stationary phases ; electrophoresis ; state
收录类别	ISTP ; SCI
原文出处	true
语种	英语
WOS记录号	WOS:000239709400002
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/97681]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Guijie,Yang, Chun,Zhang, Lihua,et al. Preparation and application of monolithic columns with narrow immobilized pH gradients[J]. talanta,2006,70(1):2-6.
APA	Zhu, Guijie,Yang, Chun,Zhang, Lihua,Liang, Zhen,Zhang, Weibing,&Zhang, Yukui.(2006).Preparation and application of monolithic columns with narrow immobilized pH gradients.talanta,70(1),2-6.
MLA	Zhu, Guijie,et al."Preparation and application of monolithic columns with narrow immobilized pH gradients".talanta 70.1(2006):2-6.