On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane

	On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane
	Yang, BC; Zhang, FF; Tian, HZ; Guan, YF
刊名	journal of chromatography a
	2006-06-09
卷号	1117 期号:2 页码:214-218
关键词	protein preconcentration capillary electrophoresis porous membrane
通讯作者	关亚风
产权排序	1;1
英文摘要	a simple on-line preconcentration method of protein for capillary electrophoresis (ce) using a cellulose acetate (ca)-coated porous membrane was proposed. ca membrane is fabricated at one of the ends of the column that allows the passage of buffer ions but excludes larger protein molecules. protein sample is continuously electrokinetically loaded and trapped by the membrane. when injection is completed, the direction of the electric field is switched and the trapped proteins are then separated by conventional ce procedure. the results achieved showed that the preconcentration mechanism of this method was based on size-exclusion effect. bovine serum albumin (bsa) was used for model protein sample, and signal enhancement of 550-fold with 15 min injection time was achieved. (c) 2006 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	dynamic ph junction ; zone-electrophoresis ; electrokinetic chromatography ; mass-spectrometry ; joint ; injection ; peptides ; analytes
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000238005800013
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/97631]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Yang, BC,Zhang, FF,Tian, HZ,et al. On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane[J]. journal of chromatography a,2006,1117(2):214-218.
APA	Yang, BC,Zhang, FF,Tian, HZ,&Guan, YF.(2006).On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane.journal of chromatography a,1117(2),214-218.
MLA	Yang, BC,et al."On-line preconcentration of protein in capillary electrophoresis with an end-column cellulose acetate-based porous membrane".journal of chromatography a 1117.2(2006):214-218.