The Cyanobacteria-Dominated Sponge Dactylospongia elegans in the South China Sea: Prokaryotic Community and Metagenomic Insights

doi:10.3389/fmicb.2017.01387

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋微生物宏基因组与分子进化研究组

	The Cyanobacteria-Dominated Sponge Dactylospongia elegans in the South China Sea: Prokaryotic Community and Metagenomic Insights
	Huang, Hui 1; Zhou, Guo-Wei 1; Gao, Zhao-Ming2 ; Wang, Yong(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Peoples R China); Wang, Yong2
刊名	FRONTIERS IN MICROBIOLOGY
	2017-07-25
卷号	8
关键词	Sponge Symbiont Cyanobacteria Poribacteria The South China Sea
DOI	10.3389/fmicb.2017.01387
产权排序	第1完成单位 ; 第2完成单位
文献子类	Article
英文摘要	The South China Sea is a special reservoir of sponges of which prokaryotic communities are less studied. Here, a new record of the sponge Dactylospongia elegans is reported near the coast of Jinqing Island in the South China Sea, and its prokaryotic community is comprehensively investigated. Sponge specimens displayed lower microbial diversity compared with surrounding seawater. At the phylum level, prokaryotic communities were consistently dominated by Proteobacteria, followed by Cyanobacteria, Chloroflexi, Acidobacteria, Actinobacteria, Gemmatimonadetes, Thaumarchaeota, and Poribacteria. Operational taxonomic unit (OTU) analysis alternatively showed that the most abundant symbiont was the sponge-specific cyanobacterial species "Candidatus Synechococcus spongiarum," followed by OTUs belonging to the unidentified Chloroflexi and Acidobacteria. Phylogenetic tree based on 16S-23S internal transcribed spacer regions indicated that the dominated cyanobacterial OTU represented a new Glade of "Ca. Synechococcus spongiarum." More reliable metagenomic data further revealed that poribacterial symbionts were highly abundant and only secondary to the cyanobacterial symbiont. One draft genome for each of the Cyanobacteria, Chloroflexi and Acidobacteria and three poribacterial genomes were extracted from the metagenomes. Among them, genomes affiliated with the Chloroflexi and Acidobacteria were reported for the first time in sponge symbionts. Eukaryotic-like domains were found in all the binned genomes, indicating their potential symbiotic roles with the sponge host. The high quality of the six recovered genomes of sponge symbionts from the sponge D. elegans makes it possible to understand their symbiotic roles and interactions with the sponge host as well as among one another.
URL标识	查看原文
WOS关键词	GENERATION SEQUENCING TECHNOLOGIES ; MICROBIAL ABUNDANCE SPONGES ; CELL GENOMICS REVEALS ; MARINE SPONGES ; CARTERIOSPONGIA-FOLIASCENS ; DIVERSITY ; SYMBIONTS ; BACTERIA ; PORIBACTERIA ; DISCOVERY
语种	英语
WOS记录号	WOS:000406344200001
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/4093]
专题	深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组
通讯作者	Wang, Yong(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Peoples R China); Wang, Yong
作者单位	1.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Peoples R China
推荐引用方式 GB/T 7714	Huang, Hui,Zhou, Guo-Wei,Gao, Zhao-Ming,et al. The Cyanobacteria-Dominated Sponge Dactylospongia elegans in the South China Sea: Prokaryotic Community and Metagenomic Insights[J]. FRONTIERS IN MICROBIOLOGY,2017,8.
APA	Huang, Hui,Zhou, Guo-Wei,Gao, Zhao-Ming,Wang, Yong,&Wang, Yong.(2017).The Cyanobacteria-Dominated Sponge Dactylospongia elegans in the South China Sea: Prokaryotic Community and Metagenomic Insights.FRONTIERS IN MICROBIOLOGY,8.
MLA	Huang, Hui,et al."The Cyanobacteria-Dominated Sponge Dactylospongia elegans in the South China Sea: Prokaryotic Community and Metagenomic Insights".FRONTIERS IN MICROBIOLOGY 8(2017).