Sensitivity and correlation of hypervariable regions in 16S rRNA genes in phylogenetic analysis

doi:10.1186/s12859-016-0992-y

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋微生物宏基因组与分子进化研究组

	Sensitivity and correlation of hypervariable regions in 16S rRNA genes in phylogenetic analysis
	Yang, Bo 1; Wang, Yong2 ; Qian, Pei-Yuan 1
刊名	BMC BIOINFORMATICS
	2016-03-18
卷号	17
关键词	16s Rrna 16s Rrna Gene Variable Regions Phylogenetic Geodesic Distance Primer
DOI	10.1186/s12859-016-0992-y
文献子类	Article
英文摘要	Background: Prokaryotic 16S ribosomal RNA (rRNA) sequences are widely used in environmental microbiology and molecular evolution as reliable markers for the taxonomic classification and phylogenetic analysis of microbes. Restricted by current sequencing techniques, the massive sequencing of 16S rRNA gene amplicons encompassing the full length of genes is not yet feasible. Thus, the selection of the most efficient hypervariable regions for phylogenetic analysis and taxonomic classification is still debated. In the present study, several bioinformatics tools were integrated to build an in silico pipeline to evaluate the phylogenetic sensitivity of the hypervariable regions compared with the corresponding full-length sequences.
WOS关键词	GRADIENT GEL-ELECTROPHORESIS ; CONSERVED 690 HAIRPIN ; COMPUTING GEODESIC DISTANCES ; ESCHERICHIA-COLI ; MICROBIAL COMMUNITIES ; ANGSTROM RESOLUTION ; PCR PRIMERS ; TREE SPACE ; NUCLEOTIDES ; CYANOBACTERIA
语种	英语
WOS记录号	WOS:000372487500001
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/1531]
专题	深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组
作者单位	1.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China 2.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, San Ya, Hai Nan, Peoples R China
推荐引用方式 GB/T 7714	Yang, Bo,Wang, Yong,Qian, Pei-Yuan. Sensitivity and correlation of hypervariable regions in 16S rRNA genes in phylogenetic analysis[J]. BMC BIOINFORMATICS,2016,17.
APA	Yang, Bo,Wang, Yong,&Qian, Pei-Yuan.(2016).Sensitivity and correlation of hypervariable regions in 16S rRNA genes in phylogenetic analysis.BMC BIOINFORMATICS,17.
MLA	Yang, Bo,et al."Sensitivity and correlation of hypervariable regions in 16S rRNA genes in phylogenetic analysis".BMC BIOINFORMATICS 17(2016).