Synchronized dynamics of bacterial niche-specific functions during biofilm development in a cold seep brine pool

doi:10.1111/1462-2920.12978

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋微生物宏基因组与分子进化研究组

	Synchronized dynamics of bacterial niche-specific functions during biofilm development in a cold seep brine pool
	Zhang, Xixiang 2; Wang, Yong3 ; Bougouffa, Salim 2; Tian, Renmao 1; Cao, Huiluo 1; Li, Yongxin 1; Cai, Lin 1; Wong, Yue Him 1; Zhang, Gen 1; Zhou, Guowei 1
刊名	ENVIRONMENTAL MICROBIOLOGY
	2015-10-01
卷号	17 期号:10 页码:4089-4104
DOI	10.1111/1462-2920.12978
文献子类	Article
英文摘要	The biology of biofilm in deep-sea environments is barely being explored. Here, biofilms were developed at the brine pool (characterized by limited carbon sources) and the normal bottom water adjacent to Thuwal cold seeps. Comparative metagenomics based on 50Gb datasets identified polysaccharide degradation, nitrate reduction and proteolysis as enriched functional categories for brine biofilms. The genomes of two dominant species: a novel Deltaproteobacterium and a novel Epsilonproteobacterium in the brine biofilms were reconstructed. Despite rather small genome sizes, the Deltaproteobacterium possessed enhanced polysaccharide fermentation pathways, whereas the Epsilonproteobacterium was a versatile nitrogen reactor possessing nar, nap and nif gene clusters. These metabolic functions, together with specific regulatory and hypersaline-tolerant genes, made the two bacteria unique compared with their close relatives, including those from hydrothermal vents. Moreover, these functions were regulated by biofilm development, as both the abundance and the expression level of key functional genes were higher in later stage biofilms, and co-occurrences between the two dominant bacteria were demonstrated. Collectively, unique mechanisms were revealed: (i) polysaccharides fermentation, proteolysis interacted with nitrogen cycling to form a complex chain for energy generation, and (ii) remarkably exploiting and organizing niche-specific functions would be an important strategy for biofilm-dependent adaptation to the extreme conditions.
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WOS关键词	SULFUR-OXIDIZING BACTERIUM ; COMMUNITY STRUCTURE ; MICROBIAL BIOFILMS ; SP NOV. ; SEA ; ADAPTATION ; EPSILONPROTEOBACTERIA ; POLYSACCHARIDE ; PSEUDOMONAS ; RESISTANCE
语种	英语
WOS记录号	WOS:000363448500049
内容类型	期刊论文
版本	出版稿
源URL	[http://124.16.218.2:8080/handle/183446/887]
专题	深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组
作者单位	1.Hong Kong Univ Sci & Technol, Div Life Sci, KAUST Global Partnership Program, Hong Kong, Hong Kong, Peoples R China 2.King Abdullah Univ Sci & Technol, Thuwal, Saudi Arabia 3.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xixiang,Wang, Yong,Bougouffa, Salim,et al. Synchronized dynamics of bacterial niche-specific functions during biofilm development in a cold seep brine pool[J]. ENVIRONMENTAL MICROBIOLOGY,2015,17(10):4089-4104.
APA	Zhang, Xixiang.,Wang, Yong.,Bougouffa, Salim.,Tian, Renmao.,Cao, Huiluo.,...&Qian, Pei-Yuan.(2015).Synchronized dynamics of bacterial niche-specific functions during biofilm development in a cold seep brine pool.ENVIRONMENTAL MICROBIOLOGY,17(10),4089-4104.
MLA	Zhang, Xixiang,et al."Synchronized dynamics of bacterial niche-specific functions during biofilm development in a cold seep brine pool".ENVIRONMENTAL MICROBIOLOGY 17.10(2015):4089-4104.