p38 MAPK regulates PKA alpha and CUB-serine protease in Amphibalanus amphitrite cyprids

doi:10.1038/srep14767

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海生物蛋白质组学及分子生物学研究组

	p38 MAPK regulates PKA alpha and CUB-serine protease in Amphibalanus amphitrite cyprids
	He, Li-Sheng2 ; Zhang, Gen 3; Qian, Pei-Yuan 3; Zhang, Yu 1; Xu, Ying 1; Wong, Yue Him 3
刊名	SCIENTIFIC REPORTS
	2015-10-05
卷号	5 期号:1
DOI	10.1038/srep14767
文献子类	Article
英文摘要	The MKK3-p38 MAPK pathway has been reported to mediate larval settlement in Amphibalanus (=Balanus) amphitrite. To clarify the underlying molecular mechanism, we applied label-free proteomics to analyze changes in the proteome of cyprids treated with a p38 MAPK inhibitor. The results showed that the expression levels of 80 proteins were significantly modified (p < 0.05). These differentially expressed proteins were assigned to 15 functional groups according to the KOG database and 9 pathways were significantly enriched. Further analysis revealed that p38 MAPK might regulate the energy supply and metamorphosis. Two potential regulatory proteins, CUB-serine protease and PKA alpha, were both down-regulated in expression. CUB-serine protease localized to postaxial seta 2 and 3, as well as the 4 subterminal sensilla in the antennule. Importantly, it was co-localized with the neuron transmitter serotonin in the sections, suggesting that the CUB-serine protease was present in the neural system. PKA alpha was highly expressed during the cyprid and juvenile stages, and it was co-localized with phospho-p38 MAPK (pp38 MAPK) to the cement gland, suggesting that PKA alpha might have some functions in cement glands. Overall, p38 MAPK might regulate multiple functions in A. amphitrite cyprids, including the energy supply, metamorphosis, neural system and cement glands.
WOS关键词	BARNACLE BALANUS-AMPHITRITE ; PROTEOMIC ANALYSIS ; NERVOUS-SYSTEM ; METAMORPHOSIS ; ATTACHMENT ; SETTLEMENT ; SEROTONIN ; INVOLVEMENT ; METABOLISM ; EXPRESSION
语种	英语
WOS记录号	WOS:000362175900001
内容类型	期刊论文
版本	出版稿
源URL	[http://124.16.218.2:8080/handle/183446/497]
专题	深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组
作者单位	1.Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China 2.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya 572000, Hainan, Peoples R China 3.Hong Kong Univ Sci & Technol, Sch Sci, Environm Sci Programs, Kowloon, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	He, Li-Sheng,Zhang, Gen,Qian, Pei-Yuan,et al. p38 MAPK regulates PKA alpha and CUB-serine protease in Amphibalanus amphitrite cyprids[J]. SCIENTIFIC REPORTS,2015,5(1).
APA	He, Li-Sheng,Zhang, Gen,Qian, Pei-Yuan,Zhang, Yu,Xu, Ying,&Wong, Yue Him.(2015).p38 MAPK regulates PKA alpha and CUB-serine protease in Amphibalanus amphitrite cyprids.SCIENTIFIC REPORTS,5(1).
MLA	He, Li-Sheng,et al."p38 MAPK regulates PKA alpha and CUB-serine protease in Amphibalanus amphitrite cyprids".SCIENTIFIC REPORTS 5.1(2015).