Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification | |
Yu, Bowen1; Jin, Zhaohui1; Huang, Ying1![]() | |
刊名 | EMBO REPORTS
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2018 | |
卷号 | 19期号:7页码:- |
关键词 | Small Rnas Hp1 Drosophila Binding Piwi Biogenesis Transcription Recognition Protein Genome |
ISSN号 | 1469-221X |
DOI | 10.15252/embr.201745418 |
文献子类 | Article |
英文摘要 | PIWI-interacting RNAs (piRNAs) silence transposons in germ cells to maintain genome stability and animal fertility. Rhino, a rapidly evolving heterochromatin protein 1 (HP1) family protein, binds Deadlock in a species-specific manner and so defines the piRNA-producing loci in the Drosophila genome. Here, we determine the crystal structures of Rhino-Deadlock complex in Drosophila melanogaster and simulans. In both species, one Rhino binds the N-terminal helix-hairpin-helix motif of one Deadlock protein through a novel interface formed by the beta-sheet in the Rhino chromoshadow domain. Disrupting the interface leads to infertility and transposon hyperactivation in flies. Our structural and functional experiments indicate that electrostatic repulsion at the interaction interface causes cross-species incompatibility between the sibling species. By determining the molecular architecture of this piRNA-producing machinery, we discover a novel HP1-partner interacting mode that is crucial to piRNA biogenesis and transposon silencing. We thus explain the cross-species incompatibility of two sibling species at the molecular level. |
电子版国际标准刊号 | 1469-3178 |
WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
语种 | 英语 |
WOS记录号 | WOS:000437275600004 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3479] ![]() |
专题 | 生化所2018年发文 上海生化细胞研究所_上海生科院生化细胞研究所 |
通讯作者 | Huang, Ying; Zhang, Z. Z. Zhao |
作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci,CAS Ctr Excellence Mol Cell, State Key Lab Mol Biol,Shanghai Key Lab Mol Andro, Natl Ctr Prot Sci Shanghai,Shanghai Sci Res Ctr,S, Shanghai, Peoples R China; 2.Carnegie Inst Sci, Dept Embryol, 115 W Univ Pkwy, Baltimore, MD 21210 USA; 3.Univ Massachusetts, Med Sch, Program Mol Med, Worcester, MA 01605 USA; 4.Fudan Univ, Collaborat Innovat Ctr Genet & Dev, State Key Lab Genet Engn, Dept Biochem,Inst Plant Biol,Sch Life Sci, Shanghai, Peoples R China |
推荐引用方式 GB/T 7714 | Yu, Bowen,Jin, Zhaohui,Huang, Ying,et al. Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification[J]. EMBO REPORTS,2018,19(7):-. |
APA | Yu, Bowen.,Jin, Zhaohui.,Huang, Ying.,Lin, Yu An.,Zhang, Z. Z. Zhao.,...&Ma, Jinbiao.(2018).Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification.EMBO REPORTS,19(7),-. |
MLA | Yu, Bowen,et al."Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification".EMBO REPORTS 19.7(2018):-. |
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